The <i>Sftpd</i> gene encodes for the C-type lectin glycoprotein surfactant protein D, which is secreted by pulmonary alveolar type II cells and nonciliated airway cells. The protein binds and coats bacteria and viruses to promote phagocytosis.  These mice carry a knock-out mutation of the <i>Sftpd</i> gene in which exon 2, including the translation start site, has been replaced by a PGK-NEO cassette. Mice that are homozygous for the targeted mutation are viable and fertile.  No gene product (mRNA or protein) is detected by Northern or Western blot analysis of lung tissue from homozygotes.  Knock-mice mice exhibit progressive accumulation of surfactant lipids, surfactant protein A, and surfactant protein B in the alveolar space. By 8 weeks of age, the alveolar phospholipid pool was 8-fold higher than wildtype controls and a 10-fold increase in the number of alveolar macrophages is observed. Homozygotes are mildly obese and exhibit hyperphagia, elevated energy consumption, redistribution of fat deposits and insulin resistance.  Knock-out mice have a 2 fold increase in basal levels of systemic gram negative bacterial endotoxin when compared to controls.  During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.

These SP-D knock-out mice exhibit progressive accumulation of lung surfactant lipids and proteins and are mildly obese. They are suitable for use in applications related to lung surfactant homeostasis, innate immunity and pulmonary inflammatory response.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

Typically mice are recovered in 12-16 weeks. <a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> to place an order or for more information.