These floxed mutant mice possess loxP sites flanking the entire coding region of the Shox2 gene. This strain may be useful for generating conditional mutations in applications related to developmental patterning, as well as cerebellar development.
John Cobb, University of Calgary
The Shox2 gene, an autosomal paralog of the human SHOXgene, encodes for a homeodomain transcription factor involved in developmental patterning.
These mice possess loxP sites flanking the entire coding region of the Shox2 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the entire coding region deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the early limb bud mesenchyme (see Stock No. 005584 for example), this mutant mouse strain may be useful in studies of developing proximal limb patterning.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette followed by a loxP site was inserted 160bp upstream of the start codon of the targeted gene, and another loxP site was inserted 315bp downstream of the stop codon. This construct was electroporated into 129S2/SvPas derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to mice, on an unknown genetic background, expressing FLPe recombinase to remove the FRT flanked NEO cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Denis Duboule|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Shox2flox; Shox2floxed|
|Gene Symbol and Name||Shox2, short stature homeobox 2|
|Strain of Origin||129S2/SvPas|
|Molecular Note||LoxP sites were inserted to flank the entire coding region of the locus. An FRT-flanked neo included in the vector was removed by FLP mediated recombination.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Shox2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #027286 in your Materials and Methods section.