This strain has been discontinued due to the loss of the 5' loxP site in the mutant allele. Please search the JAX Mice database for other strains which may be suitable for your studies or contact Technical Support for assistance.
Peter Nichol, University of Wisconsin
This strain has been discontinued due to the loss of the 5' loxP site in the mutant allele. The Aldh1a2 gene encodes an aldehyde dehydrogenase that catalyzes the synthesis of retinoic acid from retinaldehyde.
These mice possess loxP sites on either side of exon 4 of the targeted Aldh1a2 gene. Exon 4 encodes the nicotinamide adenine dinucleotide binding domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in mesoderm, this mutant mouse strain may be useful in studies of motor neuron innervation of limbs.
This strain has been discontinued due to the loss of the 5' loxP site in the mutant allele. A targeting vector designed by Dr. Shanthini Sockanathan (while at Columbia University) containing inversely oriented tk-NEO cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were subsequently crossed to several different mutant lines. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 2, Shanthini Sockanathan|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Aldh1a2, aldehyde dehydrogenase family 1, subfamily A2|
|Strain of Origin||Not Specified|
|Molecular Note||A loxP site was inserted 5' of exon 4 and an inversely oriented tk-neo cassette followed by a second loxP site were inserted 3' of exon 4.|