This is a CRISPR/Cas9 generated knock-out mutant of the triggering receptor expressed on myeloid cells 2 gene (Trem2). The allele has a NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17. The targeted Trem2 gene encodes for a protein that is part of a receptor signaling complex with TYRO protein tyrosine kinase binding protein, and that activates macrophages and dendritic cells immune response. Loss-of-function variants of the TREM2 gene are associated with Alzheimer's Disease.
Both heterozygous and homozygous mice are viable and fertile. As the mice are characterized, we may modify the strain description and add phenotype data.
Plasmids encoding a single guide RNA designed to introduce a knock out/deletion, into the Trem2 gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. Targeted embryos were transferred to pseudopregnant females. A targeted founder was identified by sequencing to have a NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17. This founder was bred to C57BL/6J (Stock No. 000664) to develop the colony.
|Allele Name||endonuclease-mediated mutation 2, MODEL-AD Center|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Allele Synonym(s)||Trem2 KO|
|Gene Symbol and Name||Trem2, triggering receptor expressed on myeloid cells 2|
|Strain of Origin||C57BL/6J|
|Molecular Note||The allele was generated by injecting Cas9 RNA and one guide sequence resulting in NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17.|
Both heterozygous and homozygous mice are viable and fertile.
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Alternatively, homozygous mice may be bred together.
When using the Trem2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027197 in your Materials and Methods section.