This is a CRISPR/Cas9 generated knock out mutant of the Trem2, triggering receptor expressed on myeloid cells 2, gene. The allele has a NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17. The targeted Trem2 gene encodes for a protein that is part of a receptor signaling complex with TYRO protein tyrosine kinase binding protein, and that activates macrophages and dendritic cells immune response. Loss-of-function variants of the TREM2 gene are associated with Alzheimer’s Disease. As the mice are characterized, we will modify the strain description and add phenotype data.
Plasmids encoding a single guide RNA designed to introduce a knock out/deletion, into the Trem2 gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. Targeted embryos were transferred to pseudopregnant females. A targeted founder was identified by sequencing to have a NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17. This founder was bred to C57BL/6J (Stock No. 000664) to develop the colony.
|Allele Name||endonuclease-mediated mutation 2, MODEL-AD Center|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Allele Synonym(s)||endonuclease-mediated mutation 2, MODEL-AD Center; Trem2em2Adiuj|
|Gene Symbol and Name||Trem2, triggering receptor expressed on myeloid cells 2|
|Gene Synonym(s)||TREM-2; Trem2b; Trem2c; Trem2a; Trem2b; Trem2c; triggering receptor expressed on myeloid cells 2c; Trem2a; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; PLOSL2|
|Strain of Origin||C57BL/6J|
|Molecular Note||The allele was generated by injecting Cas9 RNA and one guide sequence resulting in NHEJ-generated 175bp deletion that introduces a stop codon at amino acid 17.|
Heterozygotes may be bred to C57BL/6J (Stock No. 000664), or wildtype littermates. As the mice are characterized, we will modify the strain description if necessary and add data related to viability and fertility.
When using the Trem2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #027197 in your Materials and Methods section.
|Heterozygous or wildtype for Trem2<em2Msasn>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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