CD19creERT2 mice express tamoxifen-inducible Cre recombinase under the direction of the Cd19 promoter in B lymphocytes.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible, Null/Knockout) | Cd19 | CD19 antigen |
CD19creERT2 mice express a Cre-ERT2 fusion protein from endogenous CD19 antigen (Cd19) promoter/enhancer regions in B cells. This mutation is designed to abolish CD19 gene expression. CD19 is a phosphoglycoprotein, found on the surface of B lymphocytes, which binds to antigen receptors in order to decrease the threshold for antigen receptor-dependent stimulation.
Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. When CD19creERT2 mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences. The insertion of the Cre-ERT2 fusion protein results in a KO allele. Homozygous mice are viable and fertile. Induction of cre-mediated recombination through the CD19creERT2 knock-in allele is a rare event, occurring only in a few percent of B cells. In a model of Epstein–Barr viral infection (EBV) this model mimics the infection seen in humans where just a few B cells are infected in its initial phase.
A targeting vector was designed to insert a CreERT2 (Cre recombinase fused to an estrogen receptor ligand binding domain) coding sequence into exon 2 of the CD19 antigen (Cd19) gene. This construct, which also contains a frt-flanked neomycin selelction cassette, was electroporated into 129P2/OlaHsd-Hprtb-m3-derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reported that resulting chimeric mice were bred to C57BL/6J mice and were subsequently backcrossed to C57BL/6J mice for at least 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While one of the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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Site of Expression | B lymphocytes |
Allele Name | targeted mutation 1, Klaus Rajewsky |
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Allele Type | Targeted (Recombinase-expressing, Inducible, Null/Knockout) |
Allele Synonym(s) | CD19-; CD19-CreERT2; CD19creERT2 |
Gene Symbol and Name | Cd19, CD19 antigen |
Gene Synonym(s) | |
Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
Site of Expression | B lymphocytes |
Strain of Origin | 129P2/OlaHsd-Hprtb-m3 |
Chromosome | 7 |
Molecular Note | The cre/ERT2 fusion gene was knocked into exon 2. |
When maintaining a live colony, homozygous mice may be bred together.
When using the CD19creERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #027116 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Cd19<tm1(cre/ERT2)Rsky> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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