B6.Cg-Irf8^tm2.1Hm/J

Stock number: 027084
Product name: IRF8-EGFP
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

IRF8-EGFP KI mice may be useful for identifying IRF8-expressing cells during lymphoid and myeloid cell differentiation.

Detailed description

IRF8-EGFP KI mice have an enhanced green fluorescent protein sequence replacing the endogenous stop codon of the interferon regulatory factor 8 (Irf8) gene. IRF8 is a transcription factor involved in the regulation of lineage commitment and maturation of various hematopoietic lineages. Mice that are homozygous for this allele are viable and fertile. The insertion of the EGFP sequence did not influence the expression of IRF8 mRNA. These mice exhibit EGFP fluorescence in distinct hematopoietic lineages such as myeloid and lymphoid lineages, dendritic cell progenitors, B cells lineages, and granulocyte progenitors. Expression is absent in megakaryocytic–erythroid progenitors and basophil/mast cell-restricted progenitors.

Development

A targeting vector was designed to replace the stop codon of the interferon regulatory factor 8 (Irf8) gene with an enhanced green fluorescent protein (EFGP) sequence with a polyadenylation sequence, followed by a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into Ozgene B6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting offspring were bred to cre transgenic mice on a C57BL/6 background to remove the neo. These mice were crossed to remove the cre-expressing transgene and IRF8-EGFP mice were bred to C57BL/6J for at least 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Irf8^tm2.1Hm
Name: targeted mutation 2.1, Herbert Morse III
MGI accession ID: MGI:5618103
Generation method: Targeted
Attributes: Reporter
Synonyms: IRF8-EGFP
Marker symbol: Irf8
Marker name: interferon regulatory factor 8
Marker synonyms: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; Myls
Chromosome: 8
Strain of origin: B6.Cg-Thy1^a
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed in distinct hematopoietic lineages such as myeloid and lymphoid lineages, dendritic cell progenitors, B cells lineages, and granulocyte progenitors.
Molecular note: The targeting vector is designed to replace the stop codon of the endogenous gene with an enhanced green fluorescent protein (EFGP) sequence and polyadenylation sequence, followed by a loxP-flanked neomycin resistance (neo) cassette. Cre-mediated recombination removed the floxed neo cassette.