IRF8-EGFP KI mice may be useful for identifying IRF8-expressing cells during lymphoid and myeloid cell differentiation.
Herbert C. Morse III, Laboratory of Immunopathology, NIAID, NI
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Irf8 | interferon regulatory factor 8 |
IRF8-EGFP KI mice have an enhanced green fluorescent protein sequence replacing the endogenous stop codon of the interferon regulatory factor 8 (Irf8) gene. IRF8 is a transcription factor involved in the regulation of lineage commitment and maturation of various hematopoietic lineages. Mice that are homozygous for this allele are viable and fertile. The insertion of the EGFP sequence did not influence the expression of IRF8 mRNA. These mice exhibit EGFP fluorescence in distinct hematopoietic lineages such as myeloid and lymphoid lineages, dendritic cell progenitors, B cells lineages, and granulocyte progenitors. Expression is absent in megakaryocytic–erythroid progenitors and basophil/mast cell-restricted progenitors.
A targeting vector was designed to replace the stop codon of the interferon regulatory factor 8 (Irf8) gene with an enhanced green fluorescent protein (EFGP) sequence with a polyadenylation sequence, followed by a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into Ozgene B6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting offspring were bred to cre transgenic mice on a C57BL/6 background to remove the neo. These mice were crossed to remove the cre-expressing transgene and IRF8-EGFP mice were bred to C57BL/6J for at least 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | EGFP is expressed in distinct hematopoietic lineages such as myeloid and lymphoid lineages, dendritic cell progenitors, B cells lineages, and granulocyte progenitors. |
Allele Name | targeted mutation 2.1, Herbert Morse III |
---|---|
Allele Type | Targeted (Reporter) |
Allele Synonym(s) | IRF8-EGFP |
Gene Symbol and Name | Irf8, interferon regulatory factor 8 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in distinct hematopoietic lineages such as myeloid and lymphoid lineages, dendritic cell progenitors, B cells lineages, and granulocyte progenitors. |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 8 |
Molecular Note | The targeting vector is designed to replace the stop codon of the endogenous gene with an enhanced green fluorescent protein (EFGP) sequence and polyadenylation sequence, followed by a loxP-flanked neomycin resistance (neo) cassette. Cre-mediated recombination removed the floxed neo cassette. |
When maintaining a live colony, homozygotes may be bred together.
When using the IRF8-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #027084 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Irf8<tm2.1Hm> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.