Overview

Also Known As:mo/huZP2 Rescue, mo/huZP2 rescue

The mo/huZP2^Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.

Donating Investigator

Jurrien Dean, NIH / NIDDK

Genetic overview

Genetic Background	Generation

Zp2^tm1Dean

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Zp2	zona pellucida glycoprotein 2

Tg(ZP2*)4Dean

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

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Research Applications

Reproductive Biology Research

View All Research Applications

Details

Detailed Description

The mo/huZP2^Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This region of the N-terminal includes the gamete recognition domain necessary for sperm binding.
Although the zona pellucida appears normal in ovarian sections from female mice, human sperm does not bind to the zona pellucida. In the reciprocal strain, hu/mo Zp2^Rescue, human sperm binds to the zona. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.

Development

The full length sequence of the human ZP2 gene in BAC RP11-1023A8 was modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene. The linearized BAC was injected into FVB/N oocytes to generate transgenic animals. Mice from the founder line were crossed to mice carrying the Zp2^tm1Dean allele on a mixed background. Upon arrival, mice were bred to FVB/N for at least 1 generation to establish the colony.

Expression Data

Expressed Gene	ZP2, zona pellucida glycoprotein 2, human
Site of Expression

Control Suggestions

Approximate Controls

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Avella MA; Baibakov B; Dean J. 2014. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol 205(6):801-9PubMed: 24934154 MGI: J:215794

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Genetics

Zp2^tm1Dean

Allele Symbol: Zp2^tm1Dean

Allele Name	targeted mutation 1, Jurrien Dean
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Zp2^tm; Zp2^tm1Nih; Zp2^tm2Nih
Gene Symbol and Name	Zp2, zona pellucida glycoprotein 2
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	7
Molecular Note	Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice.
Mutations Made By	Jurrien Dean, NIH / NIDDK

Tg(ZP2*)4Dean

Allele Symbol: Tg(ZP2*)4Dean

Allele Name	transgene insertion 4, Jurrien Dean
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	mo/huZP2
Gene Symbol and Name	Tg(ZP2*)4Dean, transgene insertion 4, Jurrien Dean
Gene Synonym(s)
Promoter	ZP2, zona pellucida glycoprotein 2, human
Expressed Gene	ZP2, zona pellucida glycoprotein 2, human
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The full length sequence of the human ZP2 gene in BAC RP11-1023A8 is modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Fertility Defects
  - females only

Genotype: Zp2^tm1Dean related

Reproductive Biology Research
- Fertility Defects
  - females only

Mammalian Phenotype Terms by Genotype

References

Avella MA; Baibakov B; Dean J. 2014. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol 205(6):801-9PubMed: 24934154 MGI: J:215794

Additional - Zp2^tm1Dean related

Additional - Tg(ZP2*)4Dean related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Zp2 Alternate1
- Standard PCR:Tg(ZP2*)4Dean-alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these female mice are bred as heterozygous for the Zp2^tm1Dean allele and hemizygous for the transgene x homozygous homozygous (male). Female mice homozygous for the targeted allele and the transgene are poor breeders.
- Additional Breeding and Husbandry Support
Citation
When using the mo/huZP2 Rescue mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37592 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:mo/huZP2 Rescue, mo/huZP2 rescue

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Zp2tm1Dean

Allele Symbol: Zp2tm1Dean

Tg(ZP2*)4Dean

Allele Symbol: Tg(ZP2*)4Dean

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Zp2tm1Dean related

Mammalian Phenotype Terms by Genotype

References

Additional - Zp2tm1Dean related

Additional - Tg(ZP2*)4Dean related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Zp2^tm1Dean

Allele Symbol: Zp2^tm1Dean

Genotype: Zp2^tm1Dean related

Additional - Zp2^tm1Dean related