The mo/huZP2Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.
Jurrien Dean, NIH / NIDDK
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Zp2 | zona pellucida glycoprotein 2 |
Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
The mo/huZP2Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This region of the N-terminal includes the gamete recognition domain necessary for sperm binding.
Although the zona pellucida appears normal in ovarian sections from female mice, human sperm does not bind to the zona pellucida. In the reciprocal strain, hu/mo Zp2Rescue, human sperm binds to the zona. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.
The full length sequence of the human ZP2 gene in BAC RP11-1023A8 was modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene. The linearized BAC was injected into FVB/N oocytes to generate transgenic animals. Mice from the founder line were crossed to mice carrying the Zp2tm1Dean allele on a mixed background. Upon arrival, mice were bred to FVB/N for at least 1 generation to establish the colony.
Expressed Gene | ZP2, zona pellucida glycoprotein 2, human |
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Site of Expression |
Allele Name | targeted mutation 1, Jurrien Dean |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Zp2tm; Zp2tm1Nih; Zp2tm2Nih |
Gene Symbol and Name | Zp2, zona pellucida glycoprotein 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice. |
Mutations Made By | Jurrien Dean, NIH / NIDDK |
Allele Name | transgene insertion 4, Jurrien Dean |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | mo/huZP2 |
Gene Symbol and Name | Tg(ZP2*)4Dean, transgene insertion 4, Jurrien Dean |
Gene Synonym(s) | |
Promoter | ZP2, zona pellucida glycoprotein 2, human |
Expressed Gene | ZP2, zona pellucida glycoprotein 2, human |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The full length sequence of the human ZP2 gene in BAC RP11-1023A8 is modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene. |
While maintaining a live colony, these female mice are bred as heterozygous for the Zp2tm1Dean allele and hemizygous for the transgene x homozygous homozygous (male). Female mice homozygous for the targeted allele and the transgene are poor breeders.
When using the mo/huZP2 Rescue mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37592 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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