STOCK Zp2^tm1Dean Tg(ZP2*)4Dean/Mmjax

Stock number: 027053
Product name: mo/huZP2 Rescue
Strain synonyms: mo/huZP2 rescue
Research areas: Reproductive Biology Research

Description

The mo/huZP2^Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.

Detailed description

The mo/huZP2^Rescue strain carries a Zp2 KO allele and a human ZP2 BAC transgene in which exons 2-5 are replaced with the corresponding mouse DNA from exons 2-5. This region of the N-terminal includes the gamete recognition domain necessary for sperm binding. Although the zona pellucida appears normal in ovarian sections from female mice, human sperm does not bind to the zona pellucida. In the reciprocal strain, hu/mo Zp2^Rescue, human sperm binds to the zona. This strain may be useful for studying the role of the ZP2 N-terminus in human gamete recognition.

Development

The full length sequence of the human ZP2 gene in BAC RP11-1023A8 was modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene. The linearized BAC was injected into FVB/N oocytes to generate transgenic animals. Mice from the founder line were crossed to mice carrying the Zp2^tm1Dean allele on a mixed background. Upon arrival, mice were bred to FVB/N for at least 1 generation to establish the colony.

Allele

Symbol: Zp2^tm1Dean
Name: targeted mutation 1, Jurrien Dean
MGI accession ID: MGI:1932591
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Zp2
Marker name: zona pellucida glycoprotein 2
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice.

Allele

Symbol: Tg(ZP2*)4Dean
Name: transgene insertion 4, Jurrien Dean
MGI accession ID: MGI:5613982
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(ZP2*)4Dean
Marker name: transgene insertion 4, Jurrien Dean
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: ZP2,zona pellucida glycoprotein 2,human
Molecular note: The full length sequence of the human ZP2 gene in BAC RP11-1023A8 is modified by replacing the corresponding mouse exons encoding amino acids 18-156 (bp 1 in exon 2 to bp 150 in exon 5) for the human amino acids 22-161 (bp 1 in exon 2 to bp 152 in exon 5) at the N-terminus of gene.