Tie2-SR-B1 transgenic mice express the scavenger receptor class B, member 1 (Scarb1) under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. TThis strain may be useful for studying HDL metabolism and cholesterol trafficking in endothelial cells, and protection against atherosclerosis.
Alan Remaley, NHLBI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Tie2-SR-B1 transgenic mice express the scavenger receptor class B, member 1 (Scarb1) under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. SCARB1 is involved in the transfer of cholesterol between cells and high density lipoprotein cholesterol (HDLC). Overexpression of SCARB1 in endothelial cells results in decreased forward-facing surface of aortic lesions in mice with diet-induced atherosclerosis on the C57BL/6 background. Transfer of HDL through endothelial cells is approximately 4 fold faster from the apical to basolateral compartment than in the reverse direction. This strain may be useful for studying HDL metabolism and cholesterol trafficking in endothelial cells, and protection against atherosclerosis.
The Tie2-SR-B1 transgene contains mouse Scarb1 cDNA under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. The construct was microinjected into C57BL/6NJ fertilized oocytes. The founder mouse 14 was mated to C57BL/6NTac. Upon arrival, mice were bred to C57BL/6NJ for at least 1 generation to establish the colony.
Expressed Gene | Scarb1, scavenger receptor class B, member 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 14, Alan Remaley |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tie2-SR-B1 |
Gene Symbol and Name | Tg(Tek-Scarb1)14Arem, transgene insertion 14, Alan Remaley |
Gene Synonym(s) | |
Promoter | Tek, TEK receptor tyrosine kinase, mouse, laboratory |
Expressed Gene | Scarb1, scavenger receptor class B, member 1, mouse, laboratory |
Strain of Origin | C57BL/6NJ |
Chromosome | UN |
Molecular Note | The transgenic construct contains contains mouse Scarb1 cDNA under the control of the endothelial-specific receptor tyrosine kinase (Tek) promoter. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Tie2-SR-B1 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37626 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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