C57BL/6J-Tuba4a^em5Lutzy/J

Stock number: 027047
Product name: Tuba4a del50
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

This CRISPR/Cas9 generated mutant of the Tuba4a gene carries a 50 nucleotide deletion in the single coding exon. These mice may be useful in studies related to the cytoskeleton and amyotrophic lateral sclerosis (ALS).

Detailed description

CRISPR/cas9 endonuclease mediated genome editing of the Tuba4a, tubulin, alpha 4A, gene was used to introduce a 50 nucleotide deletion in the single coding exon. Some mutations in the human TUBA4A are associated with amyotrophic lateral sclerosis. The Tuba4a gene encodes one of the major constituents of microtubules. Homozygous mice are viable and fertile. As the mice are characterized, we will modify the strain description and add phenotype data.

Development

Plasmids encoding a signal guide RNA designed to introduce a 50 nucleotide deletion in the single coding exon of the Tuba4a gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J (Stock No. 000664) for 2 generations to develop the colony.

Allele

Symbol: Tuba4a^em5Lutzy
Name: endonuclease-mediated mutation 5, Cathy Lutz
MGI accession ID: MGI:6295426
Generation method: Endonuclease-mediated
Attributes: Not Specified
Marker symbol: Tuba4a
Marker name: tubulin, alpha 4A
Chromosome: 1
Strain of origin: C57BL/6J
Molecular note: CRISPR/Cas9 genome editing is used to introduce a 50 nucleotide deletion in the single coding exon of the gene.