These floxed mutant mice possess loxP sites flanking exon 3 of the Casp8 gene. This strain may be useful for generating conditional mutations in applications related to apoptosis, necrosis, inflammation and immunity.
Dr. Stephen Hedrick, University of California at San Diego
The Casp8 gene encodes a cysteinyl aspartate protease that is an essential part of the caspase activation cascade leading to TNFRSF6/FAS mediated and TNFRSF1A induced apoptosis. These mice possess loxP sites on either side of exon 3 of the targeted Casp8 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in B cells (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of the role of B cells in innate immunity.
A targeting vector was utilized in the construction of this mutant to insert loxP sites flanking exon 3. This construct was electroporated into unspecified 129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were backcrossed to C57BL/6 for at least 12 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Stephen M Hedrick|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Casp8, caspase 8|
|Strain of Origin||129|
|Molecular Note||Exon 3 was flanked with loxP sites by homologous recombination.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Casp8fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #027002 in your Materials and Methods section.
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