Overview

This subline of CBA/CaJ was derived from a 2002 bankstock and lacks the Grm6^nob8 mutation so does not have the diminished electroretinogram b-wave that CBA/CaJ mice have.

Genetic overview

Genetic Background	Generation

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Research Applications

Sensorineural Research
Diabetes and Obesity Research
Cancer Research
Reproductive Biology Research
Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This strain came from a 2002 crypreserved bankstock of CBA/CaJ. The Grm6^nob8 point mutation, which causes diminished b-wave amplitude in CBA/CaJ mice, arose spontaneously after 2002 so is absent from CBA/Ca2J, which have a normal b-wave (Peachey et al, 2017, Fig 2C).

CBA inbred mice are derived from a cross of an unpedigreed Bagg albino female and an early DBA progenitor male. C3H mice are descended from the same cross. The CBA inbred strain was initially bred for longevity and a low incidence of spontaneous mammary tumors (compared with C3H). Burdette and Strong reported that CBA mice were comparatively susceptible to tumor induction after a single subcutaneous injection of methylcholanthrene. The tumor types identified in this early work in CBA mice included spindle cell sarcoma, rhabdomyosarcoma, and epidermoid carcinoma. Strong and Smith reported finding benign hepatomas in aging CBA mice. Several groups confirmed this finding, and the majority of studies found a higher frequency of spontaneous hepatomas in males than in females.

CBA/CaJ mice are commonly used for leukemogenesis research because this strain has a low spontaneous incidence of leukemia but has a relatively high inducibility of myeloid leukemia in response to benzene and radiation exposure. Multiple reports using CBA, its F1 hybrids, and other strains, have indicated that deletions in a specific segment of chromosome 2 are linked to radiation and chemical induction of myeloid leukemia. This segment is reported to map to a 1 cM interval flanked by D2Mit126 and D2Mit185 which is homologous to human chromosome segment 11p11-12.

In addition, CBA/CaJ mice have been used for the assessment of cytostatic drug combination protocols and have also been utilized successfully as hosts for childhood rhabdomyosarcoma xenografts, after thymectomy and irradiation. CBA/CaJ mice carry viral proteins Mtv8, Mtv9, and Mtv14.

Male CBA/CaJ mice develop a mild adult onset diabetes-obesity syndrome that is characterized by hyperglycemia, hyperinsulinemia and insulin resistance. Pancreatic beta cells do not degenerate and circulating insulin levels remain high throughout life. Please see Stock No. 000654 for the CBA/CaJ strain.

Development

Beginning in 1920, Strong developed the CBA inbred strain from a cross of an unpedigreed Bagg albino female and an early DBA progenitor male. C3H mice are descended from the same cross. Progeny were selected for low mammary tumor incidence. Strong sent the CBA mice to Little at The Jackson Laboratory, who sent the strain to to Haldane and Gruneberg (university College, London) in 1932, who sent it to Carter (Institute of Animal Genetics, Edinburgh, Scotland) in 1947, who sent it to Green at The Jackson Laboratory in 1950. In 2002 the inbred strain CBA/CaJ was cryopreserved at generation F181. In 2016, when the Grm6^nob8 mutation was identified in the CBA/CaJ colony still maintained on the shelf, it was found that this 2002 bankstock lacked the Grm6^nob8 mutation. This Grm6⁺ 2002 bankstock was reanimated and bred for colony expansion then embryos were generated for cryopreservation within 2 generations of the thaw and this bankstock is CBA/Ca2J. Please see Stock No. 000654 for the CBA/CaJ strain that is homozygous for the Grm6^nob8 mutation.

Selected References

Peachey NS; Hasan N; FitzMaurice B; Burrill S; Pangeni G; Karst SY; Reinholdt L; Berry ML; Strobel M; Gregg RG; McCall MA; Chang B. 2017. A MISSENSE MUTATION IN GRM6 REDUCES BUT DOES NOT ELIMINATE MGLUR6 EXPRESSION OR ROD DEPOLARIZING BIPOLAR CELL FUNCTION. J Neurophysiol:jn.00888.2016PubMed: 28490646 MGI: J:240996

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Genetics

Grm6⁺

Allele Symbol: Grm6⁺

Allele Name	wild type
Allele Type	Not Specified
Allele Synonym(s)
Gene Symbol and Name	Grm6, glutamate receptor, metabotropic 6
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	11

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Grm6⁺ related

Sensorineural Research
- Eye Defects
  - control

Diabetes and Obesity Research
- Hyperglycemia
  - adult onset, males
- Obesity With Diabetes
  - males
- Hyperinsulinemia
  - adult onset, males
- Type 2 Diabetes (NIDDM)
  - adult onset, males
- Insulin Resistance
  - males
Cancer Research
- Increased Tumor Incidence
  - Hepatomas
  - Leukemia
  - Lymphomas
  - Mammary Gland Tumors
  - Mammary Gland Tumors: late onset
Reproductive Biology Research
- Meiotic Nondisjunction Studies
Research Tools
- General Purpose

Mammalian Phenotype Terms by Genotype

References

Peachey NS; Hasan N; FitzMaurice B; Burrill S; Pangeni G; Karst SY; Reinholdt L; Berry ML; Strobel M; Gregg RG; McCall MA; Chang B. 2017. A MISSENSE MUTATION IN GRM6 REDUCES BUT DOES NOT ELIMINATE MGLUR6 EXPRESSION OR ROD DEPOLARIZING BIPOLAR CELL FUNCTION. J Neurophysiol:jn.00888.2016PubMed: 28490646 MGI: J:240996

Additional - Grm6⁺ related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Mating System
- Sibling x Sibling
Appearance
- agouti
  Related Genotype: A/A
Citation
When using the CBA/Ca2J mouse strain in a publication, please include JAX stock #027000 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Inbred, 1 pair minimum will be supplied

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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