These Nkx2-2:Cre-EGFP Knockin/knockout mice may have applications in studies requiring conditional gene manipulation or lineage fate mapping in the CNS, pancreas and intestine.
Lori Sussel, Columbia University Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Nkx2-2 | NK2 homeobox 2 |
The Nkx2-2 gene encodes for a transcriptional activator that is involved in cell differentiation in the CNS, pancreas and intestine. These transgenic mice express a cre/EGFP fusion gene driven by the mouse Nkx2-2 promoter. The cre/EGFP was fused in-frame with the ATG start codon, most of exon 1 is deleted. Transgene expression and high levels of Cre recombinase activity are detected in Nkx2-2-expressing cells. Low levels of EGFP are detected by direct fluorescence and FACS. The homozygous mice are neonatal lethal due to pancreas and CNS defects. Heterozygotes are viable and fertile.
A targeting vector containing a cre/EGFP fusion gene and a FRT site flanked hygromycin cassette downstream was inserted into Nkx2-2tm1Suss (Nkx2-2LCA) by recombinase mediated cassette exchange (RMCE) fusing the cre/EGFP fusion gene in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a cre recombinase expression vector into 129S6/SvEvTac derived TL1 ES cells containing an Nkx2-2 (loxed cassette acceptor) allele. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to C57BL/6J mice. The mice were bred to ACTB-Flpe mice (on a mixed background) to excise the FRT flanked hygromycin cassette, and then backcrossed to C57BL/6J for more than 20 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP/cre, Green Fluorescent Protein/Cre recombinase fusion protein, |
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Site of Expression | Cre recombinase and EGFP are expressed in Nkx2-2-expressing cells of the central nervous system, pancreas, and intestine. |
Allele Name | targeted mutation 4.1, Lori Sussel |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Nkx2.2:CreEGFP |
Gene Symbol and Name | Nkx2-2, NK2 homeobox 2 |
Gene Synonym(s) | |
Expressed Gene | GFP/cre, Green Fluorescent Protein/Cre recombinase fusion protein, |
Site of Expression | Cre recombinase and EGFP are expressed in Nkx2-2-expressing cells of the central nervous system, pancreas, and intestine. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A cre/EGFP fusion gene and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that cre/EGFP was fused in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a cre-expression vector into ES cells containing an Nkx2-2 |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes have a neonatal lethal phenotype.
When using the Nkx2-2:Cre-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #026880 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Nkx2-2<tm4.1(cre/EGFP)Suss> |
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