Mice harboring the Tg(Tek-cre)2352Rwng (Tie2Cre) transgene express the Cre recombinase under the control of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter and may be useful for generating endothelial cell specific-targeted conditional mutations.
Dr. Simon John, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These transgenic mice express the Cre recombinase under the control of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter. Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (ED)7.5 and in the dorsal aorta by ED8.5. Especially high levels of expression are seen in head vasculature by ED9. When crossed with reporter strains (expressing beta-galactosidase or alkaline phosphatase), recombination is detected in all blood vessels and some blood cells examined at ED11.5, which indicates that Cre activity occurs in early vascular progenitor cells, endothelial cells and some hematopoietic cells. Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating endothelial cell specific-targeted conditional mutations.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing sequence encoding cre under the control of of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter was introduced into FVB/N donor eggs. Founder line 2352 was subsequently established.
In this line, animals from Stock No. 008573 were backcrossed to DBA/2J for at least 10 generations by the donating laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (E)7.5, in the dorsal aorta by E8.5 and is very strong in head vasculature by E9. |
Allele Name | transgene insertion 2352, Rong Wang |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Tg(Tek-cre)1Lfr; Tg(Tie2-Cre)2352; Tie2Cre; tie2-cre |
Gene Symbol and Name | Tg(Tek-cre)2352Rwng, transgene insertion 2352, Rong Wang |
Gene Synonym(s) | |
Promoter | Tek, TEK receptor tyrosine kinase, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (E)7.5, in the dorsal aorta by E8.5 and is very strong in head vasculature by E9. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | A transgenic construct containing sequence encoding cre under the control of of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter was introduced into FVB/N donor eggs. Founder line 2352 was subsequently established. Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (E)7.5, in the dorsal aorta by E8.5 and is very strong in head vasculature by E9. |
Mutations Made By | Christin Munkittrick |
Homozygotes and hemizygotes are viable and fertile.
When using the D2.FVB-Tg(Tek-cre)2352Rwng/SjJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #026860 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Tek-cre)2352Rwng |
Frozen Mouse Embryo | D2.FVB-Tg(Tek-cre)2352Rwng/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.FVB-Tg(Tek-cre)2352Rwng/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.FVB-Tg(Tek-cre)2352Rwng/SjJ | $3373.50 |
Frozen Mouse Embryo | D2.FVB-Tg(Tek-cre)2352Rwng/SjJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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