hCD68-GFP transgenic mice may be useful for studying monocyte mobilization, trafficking, and fate in models of acute and chronic inflammation.
David R Greaves, University of Oxford
Genetic Background | Generation |
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?+pN2F10
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Allele Type |
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Transgenic (Reporter, Inserted expressed sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
hCD68-GFP transgenic mice express an enhanced green fluorescent protein (EGFP) under the control of human CD68 antigen promoter/enhancer sequences. GFP is observed in CD115+ monocytes in blood, bone marrow and spleen. Also labeled are tissue-resident macrophages including Kupffer cells, microglia, and peritoneal, splenic, and alveolar macrophages. hCD68-GFP is expressed in 100% of bone marrow monocytes and 60% of bone marrow–derived neutrophils. Adoptively transferred CD68-GFP monocytes that traffic to sites of inflammation retain high-level GFP expression 72 hours after differentiation into macrophages in situ, allowing cell tracking during the resolution of inflammation. Hemizygotes are viable and fertile. Currently (12/2014) it is unknown if homozygous are similarly viable and fertile.
When compared with GFP expression in monocytes in B6.129P-Cx3cr1tm1Litt/J mice (Stock No. 005582) using a sterile zymosan peritonitis model, CX3CR1GFP monocytes downregulate GFP expression on differentiation into macrophages, while CD68-GFP monocytes retain high-level GFP expression as they differentiate to become mature macrophages.
The hCD68-GFP transgene was designed with an enhanced green fluorescent protein (EGFP) driven by the human CD68 antigen promoter/enhancer sequences. The transgene was microinjected into fertilized C57BL/6J oocytes. Heterozygous mice, with 4 copies of the transgene integrated into single site on Chromosome 3, were bred to C57BL/6 mice to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is observed in CD115+ monocytes in blood, bone marrow and spleen, and in tissue-resident macrophages. |
Allele Name | transgene insertion 1, David Greaves |
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Allele Type | Transgenic (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | CD68-GFP |
Gene Symbol and Name | Tg(CD68-EGFP)1Drg, transgene insertion 1, David Greaves |
Gene Synonym(s) | |
Promoter | CD68, CD68 molecule, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is observed in CD115+ monocytes in blood, bone marrow and spleen, and in tissue-resident macrophages. |
Strain of Origin | C57BL/6J |
Chromosome | 3 |
Molecular Note | This transgene was designed with an enhanced green fluorescent protein (EGFP) driven by the human CD68 antigen promoter/enhancer sequences. |
When maintaining a live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony. Currently (12/2014) it is unknown if homozygous are similarly viable and fertile.
When using the C57BL/6-Tg(CD68-EGFP)1Drg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026827 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CD68-EGFP)1Drg |
Frozen Mouse Embryo | C57BL/6-Tg(CD68-EGFP)1Drg/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(CD68-EGFP)1Drg/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(CD68-EGFP)1Drg/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(CD68-EGFP)1Drg/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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