B6.129-Kcnj10^tm1Kdmc/J

Stock number: 026826
Product name: Kir_4.1^f
Research areas: Cell Biology Research, Neurobiology Research, Research Tools

Description

Kir_4.1^f/f floxed mice may be useful for studying astrocyte potassium buffering.

Detailed description

Kir_4.1^f/f floxed mice possess loxP sites flanking the entire open reading frame of the potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) gene. Inward-rectifying Kir_4.1 channels are mediators of astrocyte potassium buffering during neuronal activity. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have the Kir_4.1 gene deleted in the cre-expressing tissues.

When bred to mice expressing Cre recombinase in astrocytes via the human glial fibrillary acidic protein promoter, offspring die prematurely and display severe ataxia and stress-induced seizures. They exhibit severe depolarization of both passive astrocytes and complex glia and impairment of both potassium and glutamate uptake. Membrane and action potential properties of CA1 pyramidal neurons, as well as basal synaptic transmission in the CA1 stratum radiatum, are unaffected, whereas spontaneous neuronal activity is reduced.

Development

A targeting vector was designed to insert loxP sites flanking the entire open reading frame of the potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10) gene. A frt-flanked neomycin (neo) resistance cassette was inserted downstream of the 3’ loxP site. The construct was electroporated into 129/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym transgenic mice (Stock No. 003946) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Resulting Kir_4.1^f/f floxed mice were bred to C57BL/6J mice for at least 5 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Kcnj10^tm1Kdmc
Name: targeted mutation 1, Ken D McCarthy
MGI accession ID: MGI:3761690
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Kcnj10
Marker name: potassium inwardly-rectifying channel, subfamily J, member 10
Chromosome: 1
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP site was inserted upstream of an exon encoding the entire reading frame and a frt-flanked neo cassette was inserted downstream of the exon. The neo cassette was removed by Flp-recombinase activity following a cross to Tg(ACTFLPe)9205Dym mice.