Nrxn2flox mice possess loxP sites flanking exon 20 of the neurexin II (Nrxn2) gene. NRXN2 encodes a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 20 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 20 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 20 of the neurexin II (Nrxn2) gene. Also, the splice site 4 upstream of exon 20 was mutated to a canonical splice acceptor sequence. The construct was electroporated into C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with C57BL/6 mice. Upon arrival, Nrxn2flox mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 4, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Nrxn2, neurexin II|
|Strain of Origin||C57BL/6|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 20 followed by a frt-flanked neomycin resistance cassette, and a second loxP site downstream of exon 20. Also, splice site 4, upstream of exon 20, was mutated to a canonical splice acceptor sequence.|
When maintaining a live colony, homozygous mice may be bred together.
When using the C57BL/6-Nrxn2tm4Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026683 in your Materials and Methods section.