Lag3 KO mice are useful when studying cellular proliferation, activation, and homeostasis of T cells, and plays a role in Treg suppressive function.
Robert Negrin, Stanford University School of Medicine
Exons 1-3 of lymphocyte-activation gene 3 (Lag3) have been replaced with a neomycin resistance gene in reverse orientation to the gene. The Lag3 gene encodes a transmembrane protein expressed in stimulated T lymphocytes and activated natural killer cells. LAG-3 has been found to negatively regulate cellular proliferation, activation, and homeostasis of T cells, and plays a role in Treg suppressive function. LAG-3 deficiency on CD4, but not CD8 T cells, enhances the effect of graft-verses-host disease. Although homozygous-null mice lacking a functional Lag3 gene appear normal in most respects, a difference is observed in the form of diminished natural killer cell activity.
A targeted mutation was developed that replaced exons 1-3 of lymphocyte-activation gene 3 (Lag3) with a neomycin resistance gene in reverse orientation to the gene. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were backcrossed at least 5 generations to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Christophe Benoist and Diane Mathis|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LAG-3-; Lag3tm1Dim|
|Gene Symbol and Name||Lag3, lymphocyte-activation gene 3|
|Site of Expression||Widespread.|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance gene replaced a genomic fragment containing exons 1-3. RT-PCR analysis on RNA derived from concanavalin A (Con A) induced splenocytes confirmed that no detectable transcript was produced from this allele.|
|Mutations Made By|| |
Christophe Benoist, Joslin Diabetes Center
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S2-Lag3tm1Doi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026644 in your Materials and Methods section.