STOCK Tg(Gcm1-cre)1Chrn/J

Stock number: 026625
Product name: Gcm1Cre
Research areas: Reproductive Biology Research, Research Tools

Description

These Gcm1Cre mice express Cre recombinase under the control of the mouse Gcm1 gene promoter. Cre recombinase expression is detected in the syncytiotrophoblast layer 2 of the placenta. The transgene is imprinted and expressed only when inherited from the male.

Detailed description

The Gcm1, encodes a DNA-binding protein with a glial cell missing (GCM) motif, that acts as transcription factor important for placental development. These transgenic mice express Cre recombinase under the control of the mouse Gcm1, glial cells missing homolog 1 (Drosophila), promoter. Cre recombinase expression is detected in the syncytiotrophoblast layer 2 of the placenta (the epithelial outer covering of the embryonic placental villi that invades the uterine wall). The transgene is imprinted and expressed only when inherited from the male. In addition, any floxed sequence inherited with the Gcm1-Cre transgene is deleted in the male gametes. The Donating Investigator reports that maternal inheritance does not permanently silence the transgene. Mice that are hemizygous for the transgene are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.

Development

A transgenic construct containing Cre recombinase under the control of the mouse Gcm1 gene promoter, was injected into fertilized B6/CBA F1 mouse eggs. Founder line 1 was subsequently established. The donating investigator reported that the mice were then backcrossed to 129Sv/Ev for ten generations (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were cryorecovered to establish the colony.

In 2016, a 27 SNP (single nucleotide polymorphism) panel analysis, covering all 19 chromosomes and the X chromosome, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. This revealed several markers throughout the genome (on 13 different chromosomes) were segregating in the cohort of tested mice. These data suggest that the mice sent to The Jackson Laboratory Repository were not fully backcrossed and are on a mixed genetic background.

Allele

Symbol: Tg(Gcm1-cre)1Chrn
Name: transgene insertion 1, Jean Charron
MGI accession ID: MGI:3841940
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Gcm1Cre
Marker symbol: Tg(Gcm1-cre)1Chrn
Marker name: transgene insertion 1, Jean Charron
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression is detected in the syncytiotrophoblast layer 2 of the placenta.
Molecular note: No details are available for this transgenic allele. Reference states that details will be described in a subsequent manuscript.