NOD.Chr9L mice may be useful when identifying new candidate genes in the Idd2 locus for the development of therapeutic avenues aimed at modulating DN T cell number for the prevention of autoimmune diseases.
Sylvie Lesage, Maisonneuve-Rosemont Hospital Research Center
NOD.Chr9L congenic mice bear homozygous B10.BR alleles on chromosome 9 in between markers rs13480141 (36.96Mb) and rs13480186 (50.32Mb). This locus overlaps with the Idd2 locus.
NOD.H2k2 mice were outcrossed to B10.BR mice and backcrossed to NOD.H2k2 mice carrying the TcrHEL3A9 transgene, encoding the alpha and beta chains of a T cell receptor (TCR) specific for hen egg lysozyme (HEL) (Stock No. 002597) for six generations. TcrHEL3A9 mice were maintained on a NOD.H2k2 background in which the H2k2 allele was derived from B10.BR mice (Stock No. 000465).
At the fifth generation mice were bred to iHEL transgenic mice expressing HEL under the rat insulin promoter. This transgenic line was developed in C57BL/6J oocytes, and was backcrossed onto the NOD.H2k2 background for at least 18 generations.
At the sixth generation, a recombination event occurred between the D9Mit129 and D9Mit328 markers, where one of the mice presented with a B10.BR allele at both
D9Mit129 and D9Mit328 markers. This mouse, with the longer B10.BR segment, was the founder of the 3A9 TCR:iHEL NOD.H2k2-Chr9L congenic strain. The other mice, bearing B10.BR alleles at only the D9Mit129 marker, were founders for the 3A9 TCR:iHEL NOD.H2k2-Chr9S congenic strain (see Stock No. 026243). The 3A9 TCR:iHEL NOD.H2k2-Chr9L mice were then outcrossed to NOD mice (Stock No. 001976). In this outcross, non-transgenic mice were selected to generate the current NOD.Chr9L mice.
When maintaining a live colony, homozygous mice may be bred together.
When using the NOD.Chr9L mouse strain in a publication, please cite the originating article(s) and include JAX stock #026624 in your Materials and Methods section.