These Rosa26SNAPCaaX reporter mice conditionally express membrane-localized SNAP-tag from the Gt(ROSA)26Sor promoter upon cre-mediated excision of a floxed stop cassette. Cells expressing the SNAP-tag can be selectively labeled through treatment with benzylguanine (BG)-linked compounds, including synthetic fluorophores.
Paul Heppenstall, EMBL
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
SNAP-tag is a mutant form of O6-alkylguanine-DNA alkyltransferase (a DNA repair protein) that can, when fused to proteins of interest, be used for specific labeling of cells. SNAP-tag reacts specifically and rapidly with benzylguanine (BG) which can be linked with a range of synthetic labels, including fluorophores. The labels transfer from BG derivatives to the SNAP-tag in an irreversible, covalent fashion, enabling fast and quantitative one-step labeling of the protein of interest with minimal off-target signal and low cellular toxicity.
These Rosa26SNAPCaaX cre reporter mice conditionally express SNAP-tag associated with a membrane-associated CaaX consensus sequence driven from the Gt(ROSA)26Sor promoter. Cre-mediated excision of a floxed stop cassette enables expression of membrane-associated SNAP-tag in the tissue(s) of interest and permits selective labeling upon application of a BG-linked compound.
Crosses with deleter and peripheral/central nervous-specific cre strains (e.g. NFH-cre, Avil-cre, Vglut2-cre) have demonstrated strong, sustained, cell-specific BG-fluorophore labeling capabilities. Labeling tolerates standard histological sample preparation and attains fluorescence intensities greater than those of more standard fluorescent protein reporters.
In addition for fluorophore labeling, applications may also include chromophore-assisted light activation (CALI) for ablation of cells, and the use of synthetic calcium or voltage sensors for neuronal activity imaging.
A targeting construct introduced the following components, in order, to intron 1 of the Gt(ROSA)26Sor gene: CAG-FRT-loxP-stop-loxP-(SNAP-tag/CaaX)-WPRE-pA-AttB-Pr-FRT-Neo-pA-AttP. The mutation was introduced via homologous recombination in A9 mixed 129P2 and B6 background embryonic stem cells. This strain was maintained on a mixed genetic background by the donating laboratory.
Expressed Gene | SNAP-tag, SNAP-tag, |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, Paul A Heppenstall |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) |
Allele Synonym(s) | Gt(ROSA)26Sortm1Embph; ROSA26SNAPCaaX |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | SNAP-tag, SNAP-tag, |
Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
Chromosome | 6 |
Molecular Note | A targeting construct introduced the following components, in order, to intron 1 of the Gt(ROSA)26Sor gene: CAG-FRT-loxP-stop-loxP-(SNAP-tag/CaaX)-WPRE-pA-AttB-Pr-FRT-Neo-pA-AttP. The SNAP-Tag construct contains a membrane associated CaaX consensus sequence. |
Homozygous and heterozygous mice are viable and fertile.
When using the STOCK Gt(ROSA)26Sortm1Phep/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026570 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm1Embph> |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1Embph>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1Embph>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1Embph>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Gt(ROSA)26Sor<tm1Embph>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.