Overview

Also Known As: Rb^fl/fl

These floxed mutant mice possess loxP sites flanking exon 19 of the Rb1 gene. This strain may be useful for generating conditional mutations in applications related to the study of the role played by Rb1 in development and tumorigenesis.

Donating Investigator

Irwin H. Gelman, Roswell Park Cancer Institute

Genetic overview

Genetic Background	Generation
	?+N3F6 (2019-11-01 00:00:00)

Rb1^tm2Brn

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Rb1	RB transcriptional corepressor 1

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Research Applications

Research Tools
Cancer Research
Neurobiology Research

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Base Price

Starting at:

$270.00 Domestic price for female

348.51 Domestic price for breeder pair

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Details

Detailed Description

The retinoblastoma 1 (Rb1) gene encodes a tumor suppressor that regulates the cell cycle and terminal differentiation for several cell types.
Widespread deletion of Rb1 results in embryonic lethality due to hematopoietic
and nervous system development defects.
These mice possess loxP sites on either side of exon 19 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 19 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the brain (under the control of the mouse Gfap gene promoter) and a Trp53 knock out strain, this mutant mouse strain may be useful in studies of medulloblastoma.

When bred to a floxed Trp53 strain and an ovarian intrabursal injection of an adenovirus expressing Cre recombinase, this mutant mouse strain may be useful in studies of ovarian cancer.

When bred to a strain with Cre recombinase expression in the prostatic epithelium (see Stock No. 026563) and an Akap12 knock out strain (see Stock No. 026228), this mutant mouse strain may be useful in studies of prostate cancer (see Stock No. 026229 for the triple mutant line).

Development

A targeting vector was used to introduce loxP sites flanking exon 19 of the targeted gene. The construct was electroporated into unspecified 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were maintained on a mixed FVB;129 background. The mutant mice were then crossed to other mutant lines on various genetic backgrounds, including C57BL/6 and DBA/2.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Marino S; Vooijs M; van Der Gulden H; Jonkers J; Berns A. 2000. Induction of medulloblastomas in p53-null mutant mice by somatic inactivation of Rb in the external granular layer cells of the cerebellum. Genes Dev 14(8):994-1004PubMed: 10783170 MGI: J:61961

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Genetics

Rb1^tm2Brn

Allele Symbol: Rb1^tm2Brn

Allele Name	targeted mutation 2, Anton Berns
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	targeted mutation 2, Anton Berns; Rb1^tm2Brn
Gene Symbol and Name	Rb1, RB transcriptional corepressor 1
Gene Synonym(s)	retinoblastoma 1; Rb-1; pp110; RB; Rb-1; pRb; OSRC; p105-Rb; PPP1R130; Rb; retinoblastoma 1
Strain of Origin	129
Chromosome	14
General Note	Animals homozygous for the conditional floxed exon 19 allele did not show any tumor predisposition phenotype, and were of normal fertility. Phenotypic Similarity to Human Syndrome: Glucagonoma J:216559 in double homozygous Rb1 and Trp53 conditional mutants.
Molecular Note	LoxP sites flanking exon 19 were introduced to the gene by homologous recombination. The targeted allele was verified by Southern blot analysis.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
Cancer Research
- Cre-lox System
  - loxP-flanked Sequences
Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Rb1^tm2Brn/Rb1^tm2Brn Tg(En2-cre)22Alj/0
involves: 129P2/OlaHsd * CD-1

cellular phenotype

abnormal neuronal precursor proliferation
- 8
- at P15, number of proliferating granule cell precursors in cerebellum is higher than in controls; at P20, proliferation in external granule layer (EGL) of vermis is increased while in the internal granule layer (IGL), higher proliferation is detected at P8

increased neuron apoptosis
- increase in apoptotic rate is seen at P15 in vermis of mutants; most apoptotic cells are found in the inner half of the external granule layer and in the inner granule layer; rate is similar at P30 and is accelerated compared to Rb1 single mutant mice
- Normal - there is no detectable Purkinje cell apoptosis

nervous system phenotype

abnormal brain interneuron morphology
- interneurons of molecular layer of cerebellum are substantially reduced in number at P20, which is most pronounced in lobules VI and VII

abnormal cerebellar cortex morphology
- numbers of granule, basket and stellate neurons are considerably reduced compared to wild-type

abnormal cerebellar granule cell morphology
- numbers are severely reduced in cerebellum

abnormal cerebellar granule layer morphology
- thickness and cellularity of the internal granule layer (IGL ) is reduced at P20 compared to wild-type; this is most pronounced in lobules VI and VII

abnormal cerebellum external granule cell layer morphology
- at P 15, thickness of the external granule layer (EGL) is greater (4-5 cells thick) relative to wild-type (1 or 2 cells thick)

abnormal neuronal precursor proliferation
- 8
- at P15, number of proliferating granule cell precursors in cerebellum is higher than in controls; at P20, proliferation in external granule layer (EGL) of vermis is increased while in the internal granule layer (IGL), higher proliferation is detected at P8

abnormal Purkinje cell morphology
- Purkinje cells are slightly irregularly arranged and have moderately enlarged somata and nuclei
- Purkinje cells in vermis appear disarranged, and occasionally show enlarged nuclei with abnormal shapes and reduced dendritic arborization

astrocytosis
- a brisk widespread astrogliosis in observed in the IGL

increased neuron apoptosis
- increase in apoptotic rate is seen at P15 in vermis of mutants; most apoptotic cells are found in the inner half of the external granule layer and in the inner granule layer; rate is similar at P30 and is accelerated compared to Rb1 single mutant mice
- Normal - there is no detectable Purkinje cell apoptosis

thin external granule cell layer
- at P20, mice have a persisten thin EGL with granule cell precursors still migrating through the molecular layer

Genotype: Rb1^tm2Brn/Rb1^tm2Brn Tg(Myh6-cre)2182Mds/Tg(Myh6-cre)2182Mds
involves: 129 * FVB

normal phenotype

no abnormal phenotype detected
- Normal - mutants are born with the expected Mendelian distribution and adult show no change in heart size, myocyte cell distribution, myocyte apoptosis, or mechanical function

Genotype: Rb1^tm2Brn/Rb1^tm2Brn
involves: 129 * BALB/c

adipose tissue phenotype

abnormal white adipose tissue morphology
- following transfection with adeno-cre, preadipocyte differentiation is almost completely blocked unlike in wild-type cells

Genotype: Rb1^tm2Brn/Rb1^tm2Brn
involves: 129

mammalian phenotype

neoplasm
- Normal - following treatment with cre-expressing adenovirus, mice do not develop lung tumors
- Normal - mice treated with a cre-expressing adenovirus exhibit normal lungs with no lesions

respiratory system phenotype
- Normal - mice treated with a cre-expressing adenovirus exhibit normal lungs with no lesions

References

Marino S; Vooijs M; van Der Gulden H; Jonkers J; Berns A. 2000. Induction of medulloblastomas in p53-null mutant mice by somatic inactivation of Rb in the external granular layer cells of the cerebellum. Genes Dev 14(8):994-1004PubMed: 10783170 MGI: J:61961

Additional - Rb1^tm2Brn related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Rb1^tm2Brn
- Probe:Rb1^tm2Brn Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Wild-type
- Wild-type x Heterozygote
Citation
When using the Rb^fl/fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #026563 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Herterozygous or wildtype for Rb1<tm2Brn>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: Rbfl/fl

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Rb1tm2Brn

Allele Symbol: Rb1tm2Brn

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Rb1tm2Brn/Rb1tm2Brn Tg(En2-cre)22Alj/0involves: 129P2/OlaHsd * CD-1

cellular phenotype

nervous system phenotype

Genotype: Rb1tm2Brn/Rb1tm2Brn Tg(Myh6-cre)2182Mds/Tg(Myh6-cre)2182Mdsinvolves: 129 * FVB

normal phenotype

Genotype: Rb1tm2Brn/Rb1tm2Brninvolves: 129 * BALB/c

adipose tissue phenotype

Genotype: Rb1tm2Brn/Rb1tm2Brninvolves: 129

mammalian phenotype

References

Additional - Rb1tm2Brn related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Rb^fl/fl

Rb1^tm2Brn

Allele Symbol: Rb1^tm2Brn

Genotype: Rb1^tm2Brn/Rb1^tm2Brn Tg(En2-cre)22Alj/0
involves: 129P2/OlaHsd * CD-1

Genotype: Rb1^tm2Brn/Rb1^tm2Brn Tg(Myh6-cre)2182Mds/Tg(Myh6-cre)2182Mds
involves: 129 * FVB

Genotype: Rb1^tm2Brn/Rb1^tm2Brn
involves: 129 * BALB/c

Genotype: Rb1^tm2Brn/Rb1^tm2Brn
involves: 129

Additional - Rb1^tm2Brn related