STOCK Rb1^tm2Brn/J

Stock number: 026563
Product name: Rb^fl/fl
Research areas: Cancer Research, Neurobiology Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 19 of the Rb1 gene. This strain may be useful for generating conditional mutations in applications related to the study of the role played by Rb1 in development and tumorigenesis.

Detailed description

The retinoblastoma 1 (Rb1) gene encodes a tumor suppressor that regulates the cell cycle and terminal differentiation for several cell types. Widespread deletion of Rb1 results in embryonic lethality due to hematopoietic and nervous system development defects. These mice possess loxP sites on either side of exon 19 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 19 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the brain (under the control of the mouse Gfap gene promoter) and a Trp53 knock out strain, this mutant mouse strain may be useful in studies of medulloblastoma.

When bred to a floxed Trp53 strain and an ovarian intrabursal injection of an adenovirus expressing Cre recombinase, this mutant mouse strain may be useful in studies of ovarian cancer.

When bred to a strain with Cre recombinase expression in the prostatic epithelium (see Stock No. 026563) and an Akap12 knock out strain (see Stock No. 026228), this mutant mouse strain may be useful in studies of prostate cancer (see Stock No. 026229 for the triple mutant line).

Development

A targeting vector was used to introduce loxP sites flanking exon 19 of the targeted gene. The construct was electroporated into unspecified 129 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were maintained on a mixed FVB;129 background. The mutant mice were then crossed to other mutant lines on various genetic backgrounds, including C57BL/6 and DBA/2. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Rb1^tm2Brn
Name: targeted mutation 2, Anton Berns
MGI accession ID: MGI:1931018
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rb1
Marker name: RB transcriptional corepressor 1
Chromosome: 14
Strain of origin: 129
Molecular note: LoxP sites flanking exon 19 were introduced to the gene by homologous recombination. The targeted allele was verified by Southern blot analysis.
General note: Animals homozygous for the conditional floxed exon 19 allele did not show any tumor predisposition phenotype, and were of normal fertility.

Phenotypic Similarity to Human Syndrome: Glucagonoma J:216559 in double homozygous Rb1 and Trp53 conditional mutants.