C57BL/6-Tg(KRT14-OVAL*)15Sika/J

Stock number: 026562
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These transgenic mice express a soluble form of chicken ovalbumin in the epidermis (keratinocytes) where it serves as a model self-antigen. K14-mOVA transgenic mice may be useful in applications related to graft-versus-host disease and peripheral immune tolerance.

Detailed description

These K14-sOVA transgenic mice express a soluble ovalbumin (sOVA) protein under the direction of the human KRT14, keratin 14, gene promoter.

Transgene expression (mRNA) levels in this K14-sOVA(15) line are higher than ovalbumin transgene mRNA levels detected in the K14-mOVA (membrane-bound ovalbumin (mOVA) fusion protein) transgenic line (Stock No. 026560).

Transgenic mice are tolerant to ovalbumin antigen and do not exhibit delayed-type hypersensitivity when primed and challenged with ovalbumin.

After intradermal injection of CD8+ T cells isolated from OT-I transgenic mice (see, for example, Stock No. 003831), K14-sOVA(15) transgenic mice lose weight and develop graft-versus-host disease-like skin and esophageal lesions, and die 4 to 5 days after adoptive transfer. The response observed in this strain is more severe than that seen in the K14-mOVA (membrane-bound ovalbumin (mOVA) fusion protein) transgenic line (Stock No. 026560).

The Donating Investigator reports that attempts to make the strain homozygous for the transgene were unsuccessful.

Development

A transgenic construct containing a soluble ovalbumin (sOVA) protein, the 100-1167 fragment, under the control of the 2.1kb human KRT14, keratin 14, gene promoter, was injected into fertilized C57BL/6 mouse eggs.

Founder line 15 was subsequently established (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Tg(KRT14-OVA*)15Sika
Name: transgene insertion 15, Stephen Katz
MGI accession ID: MGI:5638706
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(KRT14-OVA*)15Sika
Marker name: transgene insertion 15, Stephen Katz
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: OVA,ovalbumin,chicken
Molecular note: The transgenic construct contains a soluble (100-1167 fragment) form of ovalbumin (sOVA) under the control of the 2.1kb human KRT14, keratin 14, gene promoter. Founder lines 5, 15 and 17 were established. Founder line 15 express the highest level of OVA mRNA.