NSG-HLA-DQ8 mice (Stock No. 026561) are NOD.scid.Il2Rγcnull ("NSG") animals with the H2-Ab1 knock-out allele (Abo; see below) and the HLA-DQ8A transgene. NSG-HLA-DQ8 mice harbor several mutations, as described below.
NSG mice are non-obese diabetic animals (NOD/ShiLtJ) harboring the spontaneous severe combined immunodeficiency mutation (Prkdcscid) on chromosome 16 and the Il2Rγcnull mutation on the X chromosome (Il2rgtm1Wjl created by Dr. Warren J. Leonard [NIH]). NSG mice are described and available from The Jackson Laboratory Repository as Stock No. 005557.
The H2-Ab1 knock-out allele in Stock No. 026561 was originally reported to be H2-Ab1tm1Gru (created by Dr. Michael J. Grusby; while at Harvard) - harboring a forward-oriented neomycin resistance cassette that disrupts H2-Ab1 exon 2 (and made in ES cells with a deletion in the promoter region of H2-Ea). In 2018, quality control efforts at The Jackson Laboratory were completed that revealed Stock No. 026561 instead has the very similar H2-Ab1tm1Doi knock-out allele (created by Drs. Christophe Benoist and Diane Mathis; while at INSERM) - harboring a reverse-oriented neomycin resistance cassette that disrupts H2-Ab1 exon 2 (and made in ES cells with a deletion in the promoter region of H2-Ea). The Abo knock-out allele is on chromosome 17.
The HLA-DQ8A transgene (Tg(HLA-DQA1,HLA-DQB1)1Dv) was created by Dr. Chella David (Mayo Clinic). A 30kb DNA fragment containing the DQA*0301 gene from cosmid H11A and a 38kb DNA fragment containing the DQB*0302 gene from cosmid X10A were microinjected into (CBA/J x B10.M)F2 embryos. To establish germline transmission, founders were mated to the B10.M strain. Next, HLA-DQ8A transgenic mice were mated to NSG mice (Stock No. 005557) for at least ten generations. Subsequently, they were intercrossed with NSG mice lacking mouse MHC class II (NSG-Abo; Stock No. 021885). The resulting NSG-Abo HLA-DQ8A mice, called NSG-HLA-DQ8, are available as Stock No. 026561.
