This gene trap mutation abolishes endogenous Kif1b gene function while expressing a B-geo reporter fusion protein from the disrupted locus. Homozygous deletion of Kif1b results in early embryonic lethality. This strain may be useful for studying neural development and neurodegenerative diseases.
William G. Kaelin, Dana Farber Cancer Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Inserted expressed sequence) | Kif1b | kinesin family member 1B |
Kif1b (kinesin family member 1B) encodes an N-terminal-type motor protein and is a candidate tumor-suppressor gene involved in the pathogenesis of neuroblastoma. This gene trap mutation abolishes endogenous Kif1b gene function while expressing a B-geo reporter fusion protein from the disrupted locus. Homozygous deletion of Kif1b results in early embryonic lethality. Heterozygotes are grossly normal. Mutant mice may be useful as a lacZ reporter for Kif1b expression or as a knockout model for studying neural development and neurodegenerative diseases.
Bay Genomics gene trap embryonic stem (ES) cell line XC445 is derived from 129P2/OlaHsd and expresses B-geo (a fusion of B-galactosidase and neomycin phospotransferase II). ES cells were cultured and microinjected into blastocysts. Resultant chimeras were crossed to C57BL/6J and then backcrossed for at least 15 generations by the donating laboratory.
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
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Site of Expression |
Allele Name | gene trap XC445, BayGenomics |
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Allele Type | Gene trapped (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Kif1b, kinesin family member 1B |
Gene Synonym(s) | |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | BayGenomics gene trap vectors carry a splice acceptor sequence upstream of a Beta-geo reporter gene. Trapped gene association determined by genome coordinate overlap and strand. |
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
When using the B6.129P2-Kif1bGt(XC445)Byg/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37544 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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