Mfn2loxP floxed mice may be useful for generating conditional mutations in applications related to the study of mitochondrial fusion and morphology.
David C Chan, California Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Mfn2 | mitofusin 2 |
Mfn2loxP floxed mice possess loxP sites flanking exon 6 of the mitofusin 2 (Mfn2) gene. Mitofusin 2 encodes a transmembrane GTPase which mediates mitochondrial fusion. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 6 of the mitofusin 2 (Mfn2) gene. A second loxP site followed by a frt-flanked neomycin resistance (neo) cassette was inserted downstream of exon 6 . The construct was electroporated into 129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to Black Swiss mice. Offspring were bred with Gt(ROSA)26Sortm1(FLP1)Dym transgenic mice on a 129 background to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The donating investigator reported that Mfn2loxP mice were further backcrossed to C57BL/6J mice (Stock No. 000664) for at least 10 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 3, David C Chan |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mfn2loxP |
Gene Symbol and Name | Mfn2, mitofusin 2 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 4 |
Molecular Note | FRT-flanked neo cassette followed by two loxP sites flanking the exon 6 encoding the canonical G-1 GTPase motif replaced the endogenous locus. The neo cassette was removed by crossing with FLP recombinase expressing mice. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129(Cg)-Mfn2tm3Dcc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026525 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mfn2<tm3Dcc> |
Frozen Mouse Embryo | B6.129(Cg)-Mfn2<tm3Dcc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Mfn2<tm3Dcc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Mfn2<tm3Dcc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Mfn2<tm3Dcc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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