C57BL/6-Tg(Hspa2-hrGFP)F32Eddy/J

Stock number: 026405

Description

Transgenic Hspa2-GFP mice express Green Fluorescent Protein in spermatocytes and spermatids. This strain has applications in the isolation of spermatocytes and spermatids.

Detailed description

These mice express Green Fluorescent Protein under the control of the mouse Hspa2 gene promoter, with GFP detected in developing male germ cells. Transgene expression (mRNA) is detected in testis and at low levels in the brain. Expression of the transgene mimics the expression pattern of the endogenous HSPA2 protein. Direct green fluorescence is detected in intact testis by 506nm wavelength fluorescent light. Mice hemizygous for the Hspa2-GFP transgene are viable and fertile. Homozygotes are viable. The Donating Investigator reports that there is no difference between the phenotype exhibited by homozygotes compared to hemizygotes.

Development

The Hspa2-GFP transgene was designed with (from 5' to 3') a 53 bp mouse Acrv1 insulator element, the mouse Hspa2 promoter, the hrGFP II humanized recombinant Green Fluorescent Protein variant, polyA sequence, and another 53 bp mouse Acrv1 insulator element. This transgene was injected into C57BL/6 fertilized mouse eggs. Founder line F32 was subsequently established. The hemizygous founder male mouse was bred to a C57BL/6Ncrl female mouse. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Tg(Hspa2-hrGFP)F32Eddy
Name: transgene insertion F32, Edward M Eddy
MGI accession ID: MGI:5568248
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Hspa2-hrGFP)F32Eddy
Marker name: transgene insertion F32, Edward M Eddy
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: hrGFP,humanized Renilla Green Fluorescent Protein,
Molecular note: The transgene was designed with (from 5' to 3') a 53 bp mouse Acrv1 insulator element, the mouse Hspa2 (heat shock protein 2) promoter, the humanized renilla (hr) green fluorescent protein, polyA sequence, and another 53 bp mouse Acrv1 insulator element. Analysis by Western blotindicates that GFP is found in testes and at low levels in the brain. GFP expression is limited to meiotic and post-meiotic germ cells.