These Abr knock-out mice appear outwardly normal but have been found to be more sensitive to cockroach allergen induced asthma than wildtype mice.
Nora Heisterkamp, Childrens Hospital Los Angeles
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Abr | active BCR-related gene |
The Abr, active BCR-related gene, encodes a small GTPase that is homologous the product of Bcr gene. The expression pattern and function of the ABR protein is similar to that of the BCR protein.
These mice carry a knock-out allele of the Abr gene in which exon 3 has been replaced by a PGK-NEO cassette. No ABR protein is detected by immunoblot analysis of cerebellar extracts from homozygous mice. Homozygotes are more sensitive to cockroach allergen-induced asthma when compared to wildtype controls. Mice that are homozygous for the targeted mutation are viable and fertile.
When crossed to Bcr knock-out mice (Stock No. 026396) the resulting double mutant mice exhibit hyperactivity, circling, impaired balance and coordination, defective cerebellar foliation, impaired spatial and object recognition memory, and are more sensitive to endotoxin and hypoxia-induced pulmonary hypertension than the single knock-outs.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a PGK-NEO cassette was used to disrupt exon 3. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with Black Swiss female mice. The mice were then backcrossed to FVB/N for 12 generations.
Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from FVB/NJ female mice (Stock No. 001800). The mutant mice were then bred together to maintain the colony.
Allele Name | targeted mutation 1, John H Groffen |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Abr -/1 |
Gene Symbol and Name | Abr, active BCR-related gene |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A neomycin selection cassette was inserted into exon 3. Immunoblotting confirmed the absence of the gene product in homozygous mice. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the FVB.129(Cg)-Abrtm1Jhg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026395 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Abr<tm1Jhg> |
Frozen Mouse Embryo | FVB.129(Cg)-Abr<tm1Jhg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129(Cg)-Abr<tm1Jhg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129(Cg)-Abr<tm1Jhg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.129(Cg)-Abr<tm1Jhg>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.