Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from cryorecovery.
ReaChR protein (a red-shifted channelrhodopsin) was tagged with mCitrine fluorescent protein and placed under the conditional control of the Gt(ROSA)26Sor promoter. Excision of a single loxP- flanked stop cassette enables fluorescent expression. As an optogenetic tool, this strain is useful for the selective activation and mapping of neuron populations.Karel Svoboda, Janelia Farm Research Campus
Genetic Background | Generation |
---|---|
N2+pN2F10
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
Optogenetics enables the controlled excitation of defined neuron populations in animals that have been genetically sensitized to light. The ReaChR channelrhodopsin variant used in such studies incorporates portions of VChR1 and VChR2 (channelrhodopsins from Volvox carteri), ChIEF (the transmembrane domains of Chlamydomonas reinhardtii channelrhodopsins 1 and 2 modified with an Ile to Val mutation), and a point mutation (L171I) to enhance activation by red light (~590 nm). Axons expressing the protein become selectively inactivated during continuous excitation.
Although ReaChR viral vector models for neurological circuit mapping exist, they demonstrate some variability in expression around the injection site. To achieve more uniform expression, ReaChR tagged with fluorescent protein mCitrine was integrated into the mouse genome. Conditional expression from the Gt(ROSA)26Sor promoter is enabled upon excision of a single loxP-flanked stop cassette.
In vitro recording of coronal sections from Flp-excised animals infected with AAV-Syn-iCre (virus expressing iCre from a synapsin promoter) reveal uniform current responses from L2/3 pyramidal neurons stimulated with 590 nm light.
Applications for cortical circuit mapping with dual photostimulation (2CRACM) have been demonstrated.
A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT-flanked 3PA STOP cassette, loxP-flanked 3PA STOP cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), polyA (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene via targeted recombination in C57BL/6J x 129S6 F1-derived embryonic stem (ES) cells. Resultant animals were bred to CAGGS-FLPe mice (backcrossed N11 onto C57BL/6J; see Stock No. 003946) to excise the FRT-flanked 3PA STOP cassette. The neomycin cassette was removed by PhiC31. The resulting "Rosa26 Cag lsl ReaChR-citrine" mice were backcrossed to C57BL/6J for two generations by the donating laboratory.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
---|---|
Site of Expression | The fluorescent protein mCitrine will be expressed in cells/tissues where promoters driving Cre recombinase are expressed. |
Allele Name | targeted mutation 2.2, Keral Svoboda |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | R26 LSL-CAG-LSL-ReaChR-mCit; Rosa26 CAG-LSL-ReaChR-mCit |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | The fluorescent protein mCitrine will be expressed in cells/tissues where promoters driving Cre recombinase are expressed. |
Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
Chromosome | 6 |
Molecular Note | A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT-flanked stop cassette, loxP-flanked stop cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), poly A (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neomycin cassette. Flp-mediated recombination removed the FRT-flanked stop cassette. |
Homozygotes and heterozygotes are viable and fertile.
When using the Rosa26 CAG-LSL-ReaChR-mCit mouse strain in a publication, please cite the originating article(s) and include JAX stock #026294 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor<tm2.2Ksvo> |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm2.2Ksvo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm2.2Ksvo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm2.2Ksvo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm2.2Ksvo>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.