These R26-mp53 knock-in mice conditionally express the oncogenic Trp53 mutant R172H (mp53) and EGFP from β-actin and Gt(ROSA)26Sor promoters upon Cre-mediated excision of a loxP-flanked Stop cassette.
Dr. Ruslan Medzhitov, Yale School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Dominant negative, Inserted expressed sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
A CAG promoter, loxP-flanked neomycin-Stop cassette, mouse Trp53 R172H mutant cDNA and IRES-EGFP were introduced 3' of the Gt(ROSA)26Sor gene promoter via homologous recombination in B6.Cg-Thy1a-derived Bruce 4 embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background by the donating lab.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
Site of Expression | EGFP and a mutant Trp53 will be expressed in cells where promoters driving Cre recombinase are expressed. |
Allele Name | targeted mutation 1, Ruslan Medzhltov |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Dominant negative, Inserted expressed sequence) |
Allele Synonym(s) | R26-mp53 |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory |
Site of Expression | EGFP and a mutant Trp53 will be expressed in cells where promoters driving Cre recombinase are expressed. |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 6 |
Molecular Note | The inserted construct contains a "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene, driving ubiquitous expression of the mouse cDNA with nucleotide substitutions that results in the amino acid substitution of histidine for arginine at position 172 (R172H). The construct is preceded by a floxed STOP cassette and followed by an IRES-EGFP. The R172H mutation is predicted to create a dominant-negative product that is only expressed following cre mediated recombination to remove the STOP cassette. |
Homozygotes and heterozygotes are viable and fertile.
When using the R26-mp53KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #026283 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Gt(ROSA)26Sor<tm1(CAG-Trp53*,-EGFP)Medz> |
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