B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}/J

Stock number: 026283
Product name: R26-mp53KI
Research areas: Cancer Research, Research Tools

Description

These R26-mp53 knock-in mice conditionally express the oncogenic Trp53 mutant R172H (mp53) and EGFP from β-actin and Gt(ROSA)26Sor promoters upon Cre-mediated excision of a loxP-flanked Stop cassette.

Development

A CAG promoter, loxP-flanked neomycin-Stop cassette, mouse Trp53 R172H mutant cDNA and IRES-EGFP were introduced 3' of the Gt(ROSA)26Sor gene promoter via homologous recombination in B6.Cg-Thy1^a-derived Bruce 4 embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background by the donating lab.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(CAG-Trp53*,-EGFP)Medz}
Name: targeted mutation 1, Ruslan Medzhltov
MGI accession ID: MGI:4443069
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter; Dominant negative; Inserted expressed sequence
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: B6.Cg-Thy1^a
Expressed genes: Trp53,transformation related protein 53,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: EGFP and a mutant Trp53 will be expressed in cells where promoters driving Cre recombinase are expressed.
Molecular note: The inserted construct contains a "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene, driving ubiquitous expression of the mouse cDNA with nucleotide substitutions that results in the amino acid substitution of histidine for arginine at position 172 (R172H). The construct is preceded by a floxed STOP cassette and followed by an IRES-EGFP. The R172H mutation is predicted to create a dominant-negative product that is only expressed following cre mediated recombination to remove the STOP cassette.