Lrp5f floxed mice may be useful for studying the regulation of development through the Wnt/beta-catenin signaling cascade.
Bart O Williams, Van Andel Research Institute
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Lrp5 | low density lipoprotein receptor-related protein 5 |
Lrp5f floxed mice possess loxP sites flanking exon 2 of the low density lipoprotein receptor-related protein 5 (Lrp5) gene. LRP5 is transmembrane cell surface protein that functions as a co-receptor with Frizzled. LRP5 transmits signaling through the canonical Wnt/beta-catenin signaling cascade and plays a pivotal role in development as it is essential for cell differentiation, proliferation, and migration, as well as the development of some types of cancers. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
When bred with B6.129X1-Twist2tm1.1(cre)Dor/J mice (Stock No. 008712) expressing Cre Recombinase in early mesenchyme, which contain precursors for the skeletal tissues, offspring exhibit only a slight bone defects.
When bred to mice carrying a floxed Lrp6 allele (Stock No. 026267), and subsequently bred to B6.129X1-Twist2tm1.1(cre)Dor/J mice, double knockout mice die shortly after birth, exhibiting abnormalities including reduced body size, misshaped skull and limbs.
A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked neomycin resistance (neo) cassette, in reverse orientation, followed by a second loxP site downstream of exon 2 of the low density lipoprotein receptor-related protein 5 (Lrp5) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Resulting chimeric mice were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice (Stock No. 003946) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Mice were maintained on a mixed B6 x 129 background. Upon arrival, Lrp5f mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, van Andel Research Institute |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Lrp5f |
Gene Symbol and Name | Lrp5, low density lipoprotein receptor-related protein 5 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 19 |
Molecular Note | A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the neo cassette and left exon 2 floxed. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Lrp5f mouse strain in a publication, please cite the originating article(s) and include JAX stock #026269 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lrp5<tm1.1Vari> |
Frozen Mouse Embryo | B6;129-Lrp5<tm1.1Vari>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Lrp5<tm1.1Vari>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Lrp5<tm1.1Vari>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Lrp5<tm1.1Vari>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.