Lrp5f floxed mice possess loxP sites flanking exon 2 of the low density lipoprotein receptor-related protein 5 (Lrp5) gene. LRP5 is transmembrane cell surface protein that functions as a co-receptor with Frizzled. LRP5 transmits signaling through the canonical Wnt/beta-catenin signaling cascade and plays a pivotal role in development as it is essential for cell differentiation, proliferation, and migration, as well as the development of some types of cancers. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
When bred with B6.129X1-Twist2tm1.1(cre)Dor/J mice (Stock No. 008712) expressing Cre Recombinase in early mesenchyme, which contain precursors for the skeletal tissues, offspring exhibit only a slight bone defects.
When bred to mice carrying a floxed Lrp6 allele (Stock No. 026267), and subsequently bred to B6.129X1-Twist2tm1.1(cre)Dor/J mice, double knockout mice die shortly after birth, exhibiting abnormalities including reduced body size, misshaped skull and limbs.
