The Foxp2floxΔneo allele has loxP sites flanking the DNA-binding motif (exons 12-14) of the forkhead box P2 gene. The mice allow Cre recombinase-inducible Foxp2 knockout, and may be useful for studying speech and language development during embryogenesis/neurogenesis, speech impairment, dyslexia, cognitive function/flexibility, sensorimotor integration and motor-skill learning.
Catherine A French, Champalimaud Centre for the Unknown
Simon E Fisher, Max Planck Institute for Psycholinguistics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Foxp2 | forkhead box P2 |
The Foxp2floxΔneo floxed allele has loxP sites flanking the DNA-binding motif (exons 12-14) of forkhead box P2 (Foxp2). These Foxp2floxΔneo mice allow Cre recombinase-inducible Foxp2 knockout (as shown by loss of the floxed exons at the RNA level and an absence of Foxp2 protein).
Foxp2 is a transcription factor, and disruptions of the human protein result in a severe speech and language disorder. Foxp2 is expressed in neuronal subpopulations of several brain regions during embryogenesis and in adulthood, and is also found in other areas such as the lung, gut and spinal cord. It is involved in the processes of neurite outgrowth and synaptic plasticity and is important for sensorimotor integration and motor-skill learning
Mice homozygous for the Foxp2floxΔneo allele are viable and fertile with no reported gross physical or behavioral abnormalities.
The Foxp2floxΔneo allele was created by Drs. Simon E. Fisher and Catherine A. French (while at The Wellcome Trust Centre for Human Genetics, Oxford University). A targeting vector was designed to insert a loxP site followed by a self-excising frt-flanked neomycin resistance (neo) cassette upstream of exon 12, and a second loxP site downstream of exon 14 of the forkhead box P2 gene (Foxp2) on chromosome 6. The construct was electroporated into B6.Cg-Thy1a-derived Bruce 4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6 albino females (C57BL/6J-Tyrc-2J). The offspring still harbored the self-excising frt-flanked neo cassette (Foxp2floxneo), and were therefore bred to ACTB-FLPe transgenic mice (C57BL/6J genetic background; Stock No. 005703) to delete the neo cassette. The resulting Foxp2floxΔneo mice were bred with C57BL/6J wildtype animals to remove the Flp-expressing transgene. Foxp2floxΔneo mice were bred with C57BL/6J mice for at least ten generations. The donating investigator reports that during backcrossing, the Y chromosome was fixed to the C57BL/6J genetic background. In 2015, Dr. French (Champalimaud Centre for the Unknown, Portugal) sent black males to The Jackson Laboratory. Upon arrival, males were used to cryopreserve sperm. To establish the living Foxp2floxΔneo mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).
Allele Name | targeted mutation 1.1, Simon E Fisher |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Foxp2deltaneo |
Gene Symbol and Name | Foxp2, forkhead box P2 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 6 |
Molecular Note | Flp-mediated recombination was used to remove the FLPe-neo cassette between exons 11 and 12 leaving a floxed exon 12 through 14 region. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Foxp2floxΔneo mouse strain in a publication, please cite the originating article(s) and include JAX stock #026259 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Foxp2<tm1.1Sfis> |
Frozen Mouse Embryo | B6(Cg)-Foxp2<tm1.1Sfis>/CfreJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Foxp2<tm1.1Sfis>/CfreJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Foxp2<tm1.1Sfis>/CfreJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Foxp2<tm1.1Sfis>/CfreJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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