Tbx1-GFPflox mice conditionally express a GFP-tagged TBX1 protein. TBX1, a T-box transcription factor, is associated with Velo-cardio-facial/ DiGeorge syndrome (VCFS/DGS) syndrome. This strain may be useful for studying TBX1 and it’s role in neurogenesis, inner ear and heart development.
Bernice Morrow, Albert Einstein College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These Tbx1-GFPflox mice possess a loxP-flanked neomycin cassette that prevents expression of a Tbx1 (T-box1)-GFP fusion gene. TBX1 encodes a T-box transcription factor that maps to the center of the human 22q11.2, a region associated with Velo-cardio-facial/ DiGeorge syndrome (VCFS/DGS) syndrome. TBX1 is essential for proper ear and heart development.
Mice that are homozygous for this allele are viable and fertile.
When bred to mice that express tissue-specific Cre recombinase, resulting offspring will overexpress a GFP-tagged TBX1 protein in cre-expressing tissues. Expression of a single copy of Tbx1-GFP partially rescues heart and inner ear defects in Tbx1-null embryos, however, expression of two copies results in postnatal lethality due to cardiac outflow tract defects. This strain may be useful for studying TBX1 and it’s role in neurogenesis, inner ear and heart development.
A targeting vector containing a loxP-flanked PGK neomycin cassette and triple polyA followed by a Tbx1/GFP fusion was targeted to the ROSA26 locus. The construct was electroporated into 129/Sv, C57BL/6J and SJL – derived WW6 embryonic stem (ES) cells. The resulting chimeric animals were crossed to C57BL/6 and offspring were crossed to Swiss Webster mice for 10 generations and then maintained as a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | When bred to mice that express tissue-specific Cre recombinase, resulting offspring will overexpress a GFP-tagged TBX1 protein in cre-expressing tissues. |
Allele Name | targeted mutation 1, Bernice E Morrow |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Tbx1-GFPflox |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | When bred to mice that express tissue-specific Cre recombinase, resulting offspring will overexpress a GFP-tagged TBX1 protein in cre-expressing tissues. |
Strain of Origin | STOCK 129/Sv and C57BL/6J and SJL |
Chromosome | 6 |
Molecular Note | The targeting construct inserted into the locus a splice acceptor, floxed neomycin resistance cassette with three poly adenylation sequences and a fusion of Tbx1 with GFP. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Tbx1-GFPflox mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37516 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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