These floxed mutant mice possess loxP sites flanking exon 5 of the Afap1 gene. This strain may be useful for generating conditional mutations in applications related to Src signaling and actin filament integrity.
Jess Cunnick, The Commonwealth Medical College
Youngjin Cho, The Commonwealth Medical College
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Afap1 | actin filament associated protein 1 |
The protein encoded by the Afap1 gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissues. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
When bred to a strain with Cre recombinase expression in all tissues (see Stock No. 006054 for example), this mutant mouse strain may be useful in studies of the Src signaling pathway.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into B6.Cg-Thy1a derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6J mice for 4 generations. The mice were then crossed to OzFlpE mice, on the C57BL/6J genetic background, expressing FLPe recombinase. Mice that retained the loxP site flanked exon 5 were then bred to C57BL/6J mice for 5 generations, and the FlpE allele/transgene removed. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1.1, Youngjin Cho |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Afapfl |
Gene Symbol and Name | Afap1, actin filament associated protein 1 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 5 |
Molecular Note | Exon 5 was floxed. Flp-mediated recombination removed the FRT-flanked selection cassette inserted downstream of exon 5. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Afapfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #026214 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Afap1<tm1.1Ycho> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.