These Esr1 knock-out mice exhibit infertility. Female homozygotes display elevated serum levels of testosterone, estradiol, and luteinizing hormone. They are suitable for use in applications related to the study of the estrogen hormone signalling pathway.
Kenneth Korach, LRDT, NIEHS, NIH
Genetic Background | Generation |
---|---|
?+pN1F11
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Esr1 | estrogen receptor 1 (alpha) |
Starting at:
$236.78 Domestic price for female 4-week |
473.55 Domestic price for breeder pair |
Estrogen receptor 1 is one of 2 estrogen receptors, a ligand-activated transcription factor and nuclear hormone receptor. These mice carry a knock out mutation of the Esr1 gene in which exon 3 has been deleted. Homozygotes are viable but not fertile. No gene product (protein) is detected by Western blot analysis of uterine tissue. A faint band of truncated gene product (transcript), consistent with the deletion of exon 3, is detected by RT-PCR of uterine RNA. Tissue response to estrogen and estrogen receptor alpha activity is eliminated in homozygotes.
Homozygous females exhibit increased elevated serum testosterone, estradiol, and
luteinizing hormone levels, as well as impaired mammary gland development and uterine proliferation. Male and female growth curves are similar and do not show the sexual dimorphism in growth observed in wildtype controls. Female homozygotes have increased body weights, total body fat, and bone mineral density than wildtype female mice.
A targeting vector containing floxed TK-neo selection cassette was inserted downstream of exon 3 and a third loxP site was inserted upstream of exon 3. The construct was electroporated into unspecified C57BL/6NTac derived embryonic stem (ES) cell line. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed with mice on an unknown background and carrying the Tg(Sox2-cre)1Amc transgene to remove the floxed TK-neo cassette. The mice were the backcrossed to C57BL/6NCrl using a marker assisted protocol. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
Allele Name | targeted mutation 4.2, Kenneth S Korach |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ex3alphaERKO; Ex3-ERKO |
Gene Symbol and Name | Esr1, estrogen receptor 1 (alpha) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac |
Chromosome | 10 |
Molecular Note | A floxed TK-neo selection cassette was inserted downstream of exon 3 and a third loxP site was inserted upstream. The selection cassette and exon 3 were subsequently removed from properly targeted mice by cre excision. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304). Homozygotes are viable but not fertile.
When using the B6N(Cg)-Esr1tm4.2Ksk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026176 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Esr1<tm4.2Ksk> |
Frozen Mouse Embryo | B6N(Cg)-Esr1<tm4.2Ksk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N(Cg)-Esr1<tm4.2Ksk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N(Cg)-Esr1<tm4.2Ksk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N(Cg)-Esr1<tm4.2Ksk>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.