B6(SJL)-Adamts9^tm1.1Apte/J

Stock number: 026103
Product name: Adamts9^fl
Research areas: Metabolism Research, Research Tools

Description

Adamts9^fl/fl floxed mice may be useful for studying the regulation and consequences of extracellular matrix proteoglycans cleavage during development.

Detailed description

Adamts9^fl/fl floxed mice possess loxP sites flanking exons 5-8 of the Adamts9 gene. ADAMTS9 is a secreted metalloprotease involved in the cleavage of the extracellular matrix proteoglycan, versican, during development, although other substrates may exist. ADAMTS9 plays a role in normal gastrulation, craniofacial, cardiovascular and limb development, and melanoblast colonization of skin. Mice that are homozygous for this allele are viable and fertile.

When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-8 deleted in the cre-expressing tissues.
For example, when bred to 129S/Sv-Tg(Prm-cre)58Og/J mice (Stock No. 003328), offspring made homozygous for the deleted allele (Adamts9^del/del) exhibit cardiac valve anomalies and eye defect.
When bred to B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584), expressing Cre recombinase in early limb bud mesenchyme, resulting offspring exhibit syndactyly with interdigital webbing.

Development

A targeting vector was designed to insert a loxP- and frt-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 8 of the a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 (Adamts9) gene. The construct was electroporated into C57BL/6NTac-derived ITL IC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric males were bred to C57BL/6 females. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These Adamts9^fl/fl mice were maintained on a C57BL/6 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Adamts9^tm1.1Apte
Name: targeted mutation 1.1, Suneel Apte
MGI accession ID: MGI:5603199
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Adamts9
Marker name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9
Chromosome: 6
Strain of origin: C57BL/6NTac
Molecular note: A targeting vector was designed to insert a loxP- and FRT-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 8. FLPe-mediated recombination removed the FRT-flanked cassette leaving exons 5 through 8 floxed.