Adamts9fl/fl floxed mice may be useful for studying the regulation and consequences of extracellular matrix proteoglycans cleavage during development.
Suneel S. Apte, Cleveland Clinic
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Adamts9 | a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 |
Adamts9fl/fl floxed mice possess loxP sites flanking exons 5-8 of the Adamts9 gene. ADAMTS9 is a secreted metalloprotease involved in the cleavage of the extracellular matrix proteoglycan, versican, during development, although other substrates may exist. ADAMTS9 plays a role in normal gastrulation, craniofacial, cardiovascular and limb development, and melanoblast colonization of skin. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-8 deleted in the cre-expressing tissues.
For example, when bred to 129S/Sv-Tg(Prm-cre)58Og/J mice (Stock No. 003328), offspring made homozygous for the deleted allele (Adamts9del/del) exhibit cardiac valve anomalies and eye defect.
When bred to B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584), expressing Cre recombinase in early limb bud mesenchyme, resulting offspring exhibit syndactyly with interdigital webbing.
A targeting vector was designed to insert a loxP- and frt-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 8 of the a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 (Adamts9) gene. The construct was electroporated into C57BL/6NTac-derived ITL IC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric males were bred to C57BL/6 females. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. These Adamts9fl/fl mice were maintained on a C57BL/6 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Suneel Apte |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Adamts9fl |
Gene Symbol and Name | Adamts9, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac |
Chromosome | 6 |
Molecular Note | A targeting vector was designed to insert a loxP- and FRT-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 8. FLPe-mediated recombination removed the FRT-flanked cassette leaving exons 5 through 8 floxed. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6(SJL)-Adamts9tm1.1Apte/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026103 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Adamts9<tm1.1Apte> |
Frozen Mouse Embryo | B6(SJL)-Adamts9<tm1.1Apte>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(SJL)-Adamts9<tm1.1Apte>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(SJL)-Adamts9<tm1.1Apte>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(SJL)-Adamts9<tm1.1Apte>/J Frozen Embryo | $3373.50 |
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