Ltf-iCre knockin/knockout mice express a codon optimized Cre recombinase and EGFP under the direction of the Ltf gene. These mice are suitable for applications requiring Cre-mediated recombination in uterine epithelium.
Takiko Daikoku, Cincinnati Children's Hospital Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Ltf | lactotransferrin |
In Ltf-iCre mice, portions of exon 1 and intron 1 of the Ltf (lactotransferrin) gene are replaced by a codon optimized Cre recombinase and EGFP. The presence of iCre and EGFP abolishes expression of Ltf. LTF is a non-heme iron-binding glycoprotein expressed in the uterine epithelium of adult mice. LTF is highly responsive to estrogen in the mouse uterus. Homozygous mice are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the uterine epithelium in adult females, and in immature females after estrogen treatment. EGFP expression is observed in the uterine epithelium of mature mice. Cre activity is highest in the uterine epithelium with some activity also found in neutrophils, spleen, and esophagus.
A targeting construct was designed to replace part of exon1 and intron 1 of the Ltf (lactotransferrin) gene with a codon optimized Cre recombinase gene (iCre) followed by an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), a polyadenylation site, and a frt-flanked neomycin/kanamycin resistance (neo/kan) cassette. This construct was electroporated into (129S6/SvEvTac x C57BL/6NCr)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6(Cg)-Tyrc-2J/J blastocysts and the resulting chimeric males were bred to B6(Cg)-Tyrc-2J/J females for two generations. These Ltf-iCre mice were maintained on a mixed B6;129 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony, and the Tyrc-2J allele was bred out of the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP expression is observed in the uterine epithelium of mature mice. |
Allele Name | targeted mutation 1, Takiko Daikoku |
---|---|
Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Ltftm1(icre,EGFP)Tdku; Ltf-iCre |
Gene Symbol and Name | Ltf, lactotransferrin |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | EGFP expression is observed in the uterine epithelium of mature mice. |
Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Chromosome | 9 |
Molecular Note | A targeting construct was designed to replace part of exon1 and intron 1 of the gene with a codon optimized Cre recombinase gene (iCre) followed by an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), a polyadenylation site, and a frt-flanked neomycin/kanamycin resistance (neo/kan) cassette. |
When maintaining a live colony homozygous mice may be bred together.
When using the STOCK Ltftm1(icre)Tdku/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026030 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ltf<tm1(icre,EGFP)Tdku> |
Frozen Mouse Embryo | STOCK Ltf<tm1(icre)Tdku>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ltf<tm1(icre)Tdku>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Ltf<tm1(icre)Tdku>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Ltf<tm1(icre)Tdku>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.