Exon 4 of the Fzd6 gene is flanked by loxP sites in this conditional knockout strain. Cre-mediated excision of the floxed region appears to produce a null allele based on a pattern of hair mis-orientation.
Jeremy Nathans, Johns Hopkins University
Fzd6 (frizzled homolog 6 (Drosophila); also called Fz6) is a member of the frizzled family of genes that encode receptors for Wnt signaling proteins.
This Fzd6 conditional allele functions as a wildtype allele in the absence of Cre-mediated recombination. Exon 4 is flanked by loxP sites. Upon germline Cre-mediated recombination, the deletion allele appears to be a null allele as judged by the pattern of hair mis-orientation, which is indistinguishable from the pattern seen with a conventional null allele.
LoxP sites were inserted into introns 3 and 4 of the Fzd6 gene, flanking exon 4 (the largest coding exon in the gene). A Frt-neo-Frt cassette that was used for positive selection was introduced adjacent to the loxP site in intron 3. The targeting construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice. The Frt-flanked neo was removed by germline Flp-mediated recombination. This strain was backcrossed to C57BL/6 for two generations by the donating lab.
|Allele Name||targeted mutation 2.1, Jeremy Nathans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Fz6 CKO|
|Gene Symbol and Name||Fzd6, frizzled class receptor 6|
|Strain of Origin||129|
|Molecular Note||LoxP sites were inserted into introns 3 and 4 in order to flank exon 4 (the largest coding exon in the gene). A FRT-neo-FRT cassette that was used for positive selection was introduced adjacent to the loxP site in intron 3. FLP-mediated recombination removed the FRT-flanked neo cassette.|
Heterozygotes and homozygotes are viable and fertile.
When using the STOCK Fzd6tm2.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026008 in your Materials and Methods section.