This reduced recombination, nuclear-localized GFP reporter strain (R26 mLSL-nGFP) enables extremely sparse cre-mediated labeling of cells and is appropriate for applications such as lineage tracing.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Gt(ROSA)26Sor (R26) mLSL-nGFP mice incorporate a nuclear-localization signal (nls)-GFP open reading frame inserted downstream of a loxP-stop-loxP cassette in which one loxP site is wild type and the other is mutant loxP63 (mutant-LSL or "mLSL"). The stop cassette consists of three tandem SV40 polyadenylation/transcription termination sequences.
When crossed with Cre-expressing strains, the result is reduced efficiency of Cre-mediated recombination. There is no background recombination in the absence of 4-hydroxytamoxifen (4HT) when tested with typical CreER lines. These mice will be useful for applications that require extremely sparse labeling, such as lineage tracing. Mice homozygous for this allele are viable and fertile with no obvious physical or behavioral abnormalities.
A targeting vector containing (from 5' to 3') a CAG promoter, a mutant loxP63 (ATAACTTCGTATAGCCTACATTATACGAAGTTAT)-triple poly A/stop-wild type loxP (ATAACTTCGTATAGCATACATTATACGAAGTTAT), a nuclear-localized GFP with a C-terminal 6-Myc epitope tag, a bovine growth hormone 3' UTR, and a Frt-PGKneo-Frt cassette was inserted into the Gt(ROSA)26Sor locus. The stop cassette consists of three tandem SV40 polyadenylation/transcription termination sequences. The construct was electroporated into 129-derived ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were crossed to C57BL/6 mice for two generations by the donating laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Upon induction with 4-hydroxytamoxifen (4HT) GFP is observed in Cre-expressing tissues. |
Allele Name | targeted mutation 9, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | R26 mLSL-nGFP |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Upon induction with 4-hydroxytamoxifen (4HT) GFP is observed in Cre-expressing tissues. |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | The targeting vector containins (from 5' to 3') a CAG promoter, a mutant loxP63 (ATAACTTCGTATAGCCTACATTATACGAAGTTAT)-triple poly A/stop-wild type loxP (ATAACTTCGTATAGCATACATTATACGAAGTTAT), a nuclear-localized GFP with a C-terminal 6-myc epitope tag, a bovine growth hormone 3' UTR, and a Frt-PGKneo-Frt cassette. The stop cassette consists of three tandem SV40 polyadenylation/transcription termination sequences. |
Heterozygotes and homozygotes are viable and fertile.
When using the B6;129-Gt(ROSA)26Sortm9(CAG-GFP*)Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026005 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm9(CAG-GFP*)Nat> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm9(CAG-GFP*)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm9(CAG-GFP*)Nat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm9(CAG-GFP*)Nat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm9(CAG-GFP*)Nat>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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