The CB013 embryonic stem cell line was established from a cross between a homozygous B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax (3XTg-AD) female (JAX#004807) and a male homozygous for Mapttm1(EGFP)Klt. This cell line may be useful in in vitro experiments directed towards differentiation of ESCs to cortical neurons expressing AD-relevant mutations.
Laura Reinholdt, The Jackson Laboratory
The CB013 embryonic stem cell line was established using day 3.5 blastocysts from a cross between a homozygous B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax female (MMRRC #034830/JAX#004807) and a male homozygous for Mapttm1(EGFP)Klt . This cross resulted in biological replicates that are heterozygous/hemizygous for Psen1tm1Mpm and Tg(APPSwe,tauP301L)1Lfan (MMRRC#03557, #03558). The recommended wild type control is MMRRC#037551 . All lines are heterozygous for Mapttm1(EGFP)Klt . These cell lines have been proven useful in in vitro experiments directed towards differentiation of ESCs to cortical neurons. Cell lines were derived and maintained on mitomycin-C treated mouse embryonic fibroblast feeder cells (MEFs) derived from C57BL/6J embryos. This cell line was not germline tested.
Currently there are no related genes or alleles for this strain.
When using the STOCK Mapttm1(EGFP)Klt Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa /Mmjax CB013 mES cells mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37559 in your Materials and Methods section.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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