B6(SJL)-Shcbp1^{tm1c(EUCOMM)Wtsi}/RaviJ

Stock number: 025770
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Shcbp1^fl/fl floxed mutant mice may be useful for studying the proliferative stages of T cell development and the regulation of CD4⁺ T cell effector function.

Detailed description

Shcbp1^fl/fl floxed mutant mice possess loxP sites flanking exons 4-6 of the Shc SH2-domain binding protein 1 (Shcbp1) gene. ShcBP1 binds to the adaptor protein ShcA, which is a regulator of T cell development and proliferation. ShcBP1 has also been linked to cell proliferation, embryonic development, growth factor signaling, and tumorigenesis. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 4-6 deleted in the cre-expressing tissues.

The knockout version of this allele is available as Stock No. 025771.

Development

C57BL/6N-A^tm1Brd-derived JM8A3.N1 embryonic stem (ES) cells containing a targeted mutation of the Shc SH2-domain binding protein 1 (Shcbp1) gene were obtained from The European Conditional Mouse Mutagenesis Program (EUCOMM). The Shcbp1 gene has a frt-flanked and loxP-flanked neomycin resistance (neo) cassette upstream of exon 4, and a third loxP site downstream of exon 6. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric mice were bred C57BL/6J. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. Shcbp1^fl/fl mice were maintained on a C57BL/6J background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Shcbp1^{tm1c(EUCOMM)Wtsi}
Name: targeted mutation 1c, Wellcome Trust Sanger Institute
MGI accession ID: MGI:5582589
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Shcbp1
Marker name: Shc SH2-domain binding protein 1
Chromosome: 8
Strain of origin: C57BL/6N-A^tm1Brd
Molecular note: The L1L2_Bact_P cassette was inserted at position 4750268 of Chromosome 8 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4748418. The critical exon(s) are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Actin-Flp recombinase, leaving exons 4 and 6 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.