C57BL/6J-Ripk3^m1Btlr/J

Stock number: 025738
Research areas: Apoptosis Research

Description

Ripk3^m1Btlr ENU-induced mutant mice have a mutation within the donor splice site of intron 2 of the Ripk3 gene. This strain may be useful when studying inflammation, oxidative stress and apoptosis.

Detailed description

This strain was generated by Dr. Bruce Beutler at The University of Texas Southwestern Medical Center. Ripk3^m1Btlr ENU-induced knock-out mice have a mutation within the donor splice site of intron 2 (1 bp from exon 2) of the receptor-interacting serine-threonine kinase 3 (Ripk3) gene. RIPK3 is a cytoplasmic member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases and is a trigger for pro-inflammatory apoptotic cell death. In these mice, RIP3 deletion protects against brain injury through inhibiting inflammation, oxidative stress and apoptosis, potentially making RIP3 a therapeutic target against traumatic brain injury. Homozygotes are viable and fertile.

For additional information on this mutation, please see the Mutagenetix website.

Development

Following multidose N-ethyl-N-nitrosourea (ENU) treatments to induce mutations in founder male C57BL/6J mice, whole exome sequencing of the G1 grandsire identified a C to T transition at base pair 55788237 (v38) on chromosome 14, within the donor splice site of intron 2 of Ripk3. The Ripk3^m1Btlr mice were maintained on a C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.

Allele

Symbol: Ripk3^m1Btlr
Name: mutation 1, Bruce Beutler
MGI accession ID: MGI:5705237
Generation method: Chemically induced (ENU)
Marker symbol: Ripk3
Marker name: receptor-interacting serine-threonine kinase 3
Marker synonyms: 2610528K09Rik; Hcyp2; RIP3
Chromosome: 14
Strain of origin: C57BL/6J
Molecular note: ENU-mutagenesis induced a C to T transition at base pair 55788237 (v38) on chromosome 14, within the donor splice site of intron 2