These floxed mutant mice possess loxP sites flanking the single exon (entire coding region) of the Drd5 gene. This strain may be useful for generating conditional mutations in applications related to learning and memory.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Activation of hippocampal dopamine 1-class receptor D5R (Drd5, dopamine receptor D5) is implicated in contextual fear conditioning (CFC). Robust expression of Drd5 mRNA is seen in all hippocampal subregions.
The single exon of the mouse Drd5 gene is flanked by loxP sites in this conditional targeted mutant strain. Cre-mediated excision of the floxed region produces a knockout allele.
Animals created from crosses with Pomc-Cre mice (e.g. Stock No. 010714) to create knockouts in the granule cells of the dentate gyrus do not demonstrate fear memory deficits, nor do they exhibit generalized fear between two similar but different contexts.
Animals homozygous for widespread knockouts of Drd5 receptors are viable.
A loxP site was introduced 5' of the targeted gene's single exon and an FRT-loxP-(neo-tk)-FRT-loxP (LFNT) cassette was introduced 3' of the exon. The mutation was created through homologous recombination in B6.Cg-Thy1a-derived Bruce 4 embryonic stem (ES) cells. Transfection with a plasmid carrying Cre recombinase excised the neo cassette, leaving the coding region flanked by loxP sites. Mice were then backcrossed 4-5 generations to C57BL/6 by the donating lab.
|Allele Name||targeted mutation 1.1, Susumu Tonegawa|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||D5R flx|
|Gene Symbol and Name||Drd5, dopamine receptor D5|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||A loxP site was introduced 5' of the targeted gene's single exon and an FRT-loxP-(neo-tk)-FRT-loxP (LFNT) cassette was introduced 3'. Cre-mediated recombination excised the neo cassette, leaving the coding region flanked by loxP sites.|
Homozygotes and heterozygotes are viable and fertile.
When using the B6.Cg-Drd5tm1.1Stl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025704 in your Materials and Methods section.