The Adcy3 gene encodes for a Ca2+-calmodulin-sensitive adenylate cyclase that catalyzes cAMP formation. These mice carry a chemically induced (ENU) point mutation of Adcy3: a G-to-A transition at base 827, M279I. The Jll allele is hyperactive compared to the wildtype allele, and is dominantly inherited. After 8 weeks on a high fat diet, heterozgous mice on the C57BL/6 background exhibit lower body weights and fat mass (absolute and relative) than wildtype controls. Heterozygotes on the B6;129 background also have lower fasting plasma leptin and plasma insulin levels than wildtype controls. Homozygous mice on both the B6;129 and C57BL/6 backgrounds exhibit a phenotype similar to that seen in heterozygotes. Mice that are homozygous for the targeted mutation (B6;129 and C57BL/6 backgrounds) are viable and fertile.

This ENU mutagenesis generated point mutation of Adcy3 results in a hyperactive allele, Jll, and is useful in studies of diet induced obesity, energy homeostasis, and cAMP signaling.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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