The predominantly expressed pyruvate dehydrogenase kinase isoform in skeletal muscle, isoenzyme 2, is encoded by the Pdk2 gene. Located in the mitrochondrial matrix, pyruvate dehydrogenase kinase, isoenzyme 2, inhibits the pyruvate dehydrogenase complex, and is involved in glucose metabolism regulation. These mice carry a knock out targeted mutation of the Pdk2 gene in which exon 1 and part of exon 2 are replaced by a PGK-NEO cassette. Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (protein) is detected by Western blot analysis of mitochondria isolated from muscle tissue from homozygous animals. Homozygotes exhibit higher pyruvate dehydrogenase complex activity, and reduced non-fasting blood glucose levels when compared to wildtype controls. 

 
When crossed with Pdk4 knock out mice (Stock No. <a href="https://www.jax.org/strain/025633">025633</a>), the resulting double homozygotes exhibit lower blood glucose levels, fasting induced ketoacidosis and hypothermia, and increased pyruvate dehydrogenase complex activity.

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This Pdk2 knockout strain is useful in studies of glucose homeostasis.

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