JAX Mouse Strain Datasheet - 025619

B6.Cg-Tg(CAG-GCaMP2)2Mik/J

Stock No:: 025619 |; CHROMus line pCAGGS-GCaMP2

Cryo Recovery

Overview

Also Known As: CHROMus line pCAGGS-GCaMP2

pCAGGS-GCaMP2 transgenic mice are a fluorescent calcium indicator strain that exhibits high calcium-sensor expression in widespread cells/tissues. Upon GCaMP2 binding to calcium, increased EGFP fluorescence is observed. The entire transgene is flanked with loxP sites.

Donating Investigator

Michael I Kotlikoff, Cornell University

Genetic overview

Genetic Background	Generation

Tg(CAG-GCaMP2)2Mik

Allele Type
Transgenic (Reporter)

View Genetics

Research Applications

Cancer Research
Cardiovascular Research
Cell Biology Research
Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

pCAGGS-GCaMP2 transgenic mice express the fluorescent calcium indicator GCaMP2 under control of the CAG hybrid promoter. pCAGGS-GCaMP2 transgenic mice from founder line 2 exhibit high sensor expression (GCaMP2 expression/EGFP fluorescence) in widespread cells/tissues.In the absence of calcium binding, low/baseline EGFP fluorescence is observed. Following calcium binding (such as muscle contraction, arteriolar vasodilation etc.), bright EGFP fluorescence is observed. Of note, the entire transgene is flanked with loxP sites, allowing for removal of the transgene via Cre recombinase if so desired.

Hemizygous and homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. The donating investigator reports that homozygotes have brighter fluorescence than hemizygotes.

Calcium is a key molecular signal for cell functions including heart, smooth muscle, vessel, and airway contraction, lung secretion, autonomic neurotransmission and immunocyte function. pCAGGS-GCaMP2 transgenic mice may be useful to examine calcium signaling in widespread cells/tissues both in vivo and in vitro.

The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (with several amino acid substitutions designed to increase brightness, dynamic range and thermal stability, as well as to prevent GFP dimerization), and a rat calmodulin sequence. In the absence of calcium binding, dim EGFP fluorescence is expected as there is a pore from the outside of its barrel into the chromophore. Upon calcium binding, this pore becomes occupied and fluorescence is increased.

This mouse model is available by way of a collaborative effort between Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus) and The Jackson Laboratory.

Development

pCAGGS-GCaMP2 transgenic mice were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus).
The 3.7 kbp pCAGGS-GCaMP2 transgene has a loxP site, the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), the GCaMP2 sequence (described below), a polyadenylation signal and a second loxP site.The transgene was microinjected into B6D2F2 embryos, and founder animals were bred to C57BL/6J inbred mice for germline transmission. pCAGGS-GCaMP2 founder line 2 was identified with high sensor expression. The donating investigator reports that pCAGGS-GCaMP2 transgenic mice from this founder line were backcrossed two generations to C57BL/6J wildtype mice, then bred together nine generations as homozygotes, and then backcrossed five more generations to C57BL/6J wildtype mice. In 2015, mice were sent to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To establish our living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).

Of note, the donating investigator reports that, at least once during backcrossing, a hemizygous female was bred to a C57BL/6J inbred male (thus the Y chromosome of the congenic strain is of C57BL/6J origin).

The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in increased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K).

Expression Data

Expressed Gene	GCaMP, Genetically encoded calcium indicator,
Site of Expression	EGFP expression is widespread.

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Tg(CAG-GCaMP2)2Mik

Allele Symbol: Tg(CAG-GCaMP2)2Mik

Allele Name	transgene insertion 2, Michael I Kotlikoff
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	pCAGGS-GCaMP2
Gene Symbol and Name	Tg(CAG-GCaMP2)2Mik, transgene insertion 2, Michael I Kotlikoff
Gene Synonym(s)
Promoter	CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter,
Expressed Gene	GCaMP, Genetically encoded calcium indicator,
Site of Expression	EGFP expression is widespread.
Strain of Origin	(C57BL/6 x DBA/2)F2
Chromosome	UN
Molecular Note	This 3.7 kbp transgene has a loxP site, the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), the GCaMP2 sequence (described below), a polyadenylation signal and a second loxP site. Founder line 2 was identified with high sensor expression. The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in increased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K).

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Cre-lox System
  - loxP-flanked Sequences
Cardiovascular Research
- Heart Abnormalities
- Other
Cell Biology Research
- Channel and Transporter Defects
  - calcium
Neurobiology Research
- Channel and Transporter Defects
  - calcium
- Cre-lox System
  - loxP-flanked Sequences
- Optogenetic and Chemogenetic tools
  - Calcium sensor expressing strains
Research Tools
- Cancer Research
  - Cre-lox System
- Cardiovascular Research
  - Cre-lox System
- Cell Biology Research
- Cre-lox System
  - loxP-flanked Sequences
- Developmental Biology Research
  - Cre-lox System
- Fluorescent Proteins
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: endothelial cells
- Reproductive Biology Research
  - Cre-lox System

References

Additional - Tg(CAG-GCaMP2)2Mik related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :. MGI: J:101977

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: CHROMus line pCAGGS-GCaMP2

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Tg(CAG-GCaMP2)2Mik

Allele Symbol: Tg(CAG-GCaMP2)2Mik

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Tg(CAG-GCaMP2)2Mik related

Genotyping Protocols

Breeding Considerations

Mating System

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability