JAX Mouse Strain Datasheet - 025618

B6;FVB-Tg(RP24-353L5-mCherry)5Mik/J

Stock No:: 025618 |; CHROMus line Mesp1^BAC-mCherry

Transgenic

Cryo Recovery

Overview

Also Known As: CHROMus line Mesp1^BAC-mCherry

Mesp1^BAC-mCherry transgenic mice exhibit high mCherry fluorescence in mesodermal cell lineages during early gastrulation.

Donating Investigator

Michael I Kotlikoff, Cornell University

Genetic overview

Genetic Background	Generation

Tg(RP24-353L5-mCherry)5Mik

Allele Type
Transgenic (Reporter)

View Genetics

Research Applications

Cardiovascular Research
Cell Biology Research
Developmental Biology Research
Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mesp1^BAC-mCherry transgenic mice express the red fluorescent protein mCherry under control of the Mesp1 locus promoter/enhancer regions within the BAC transgene. Mesp1^BAC-mCherry transgenic mice from founder line 5 exhibit mCherry expression (red fluorescence) in mesodermal cell lineages during early gastrulation, including cardiac mesoderm. In total, transgene expression is consistent with endogenous Mesp1.

Hemizygous and homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. The donating investigator reports that homozygotes have brighter fluorescence than hemizygotes.

This mouse model is available by way of a collaborative effort between Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus) and The Jackson Laboratory.

Development

Mesp1^BAC-mCherry transgenic mice were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus).
The ~156 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP24-353L5 was obtained; containing the entire mesoderm posterior 1 locus (Mesp1) as well as ~23 kbp centromeric flanking sequences (including a portion of the Wdr93 locus) and ~131 kbp distal flanking sequences (including the complete Mesp2, Anpep and Ap3s2 loci). Using homologous recombination/BAC recombineering, a construct containing the mCherry sequence, an SV40 polyadenylation signal, an SV40-puro-pA cassette and flanking vector sequences was all inserted into the ATG start site of the BAC Mesp1 gene (replacing the initiation codon of Mesp1 in exon 1). No other loci on the BAC were altered.The resulting ~165 kbp modified BAC (Mesp1^BAC-mCherry-pA-SV40-puro-pA) was purified and then microinjected into FVB/N embryos. Founder animals were bred to C57BL/6J inbred mice for germline transmission. The donating investigator reports that Mesp1^BAC-mCherry founder line 5 was backcrossed two generations to C57BL/6J wildtype mice, then bred together six generations as homozygotes, and then backcrossed four more generations to C57BL/6J wildtype mice. See SNP results below.In 2015, mice were sent to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To establish our living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).

Of note, the donating investigator reports that, at least once during backcrossing, a hemizygous female was bred to a C57BL/6J inbred male (thus the Y chromosome of the congenic strain is of C57BL/6J origin).

In 2015, a 32 SNP (single nucleotide polymorphism) panel analysis was performed on the two male mice first received at The Jackson Laboratory Repository. This panel has 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains. Importantly, those latter 5 markers are the same for C57BL/6N and FVB. The SNP panel revealed 4 of 27 markers were not fixed for C57BL/6 allele-type (i.e., still segregating for FVB allele-type markers); specifically 2 markers on chromosome 13 (~21 Mbp) in both mice, with each mouse also having one other C57BL/6;FVB mix marker (on chromosome 9 [~6.6 cM] or chromosome 10 [~87 Mbp]). In addition, 2 of the 5 markers that determine C57BL/6J from C57BL/6N (FVB) revealed both mice were segregating on chromosome 13 [~41.0 Mbp], with one mouse also segregating on chromosome 11 [~4 Mbp]. Collectively, these data suggest the mice sent to The Jackson Laboratory were not fully backcrossed onto C57BL/6, and are instead on a C57BL/6;FVB mixed genetic background.

Expression Data

Expressed Gene	Anpep, alanyl (membrane) aminopeptidase, mouse, laboratory
Expressed Gene	Ap3s2, adaptor-related protein complex 3, sigma 2 subunit, mouse, laboratory
Expressed Gene	Mesp1, mesoderm posterior 1, mouse, laboratory
Expressed Gene	Mesp2, mesoderm posterior 2, mouse, laboratory
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	mCherry is expressed in mesodermal cell lineages during early gastrulation.

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Tg(RP24-353L5-mCherry)5Mik

Allele Symbol: Tg(RP24-353L5-mCherry)5Mik

Allele Name	transgene insertion 5, Michael I Kotlikoff
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	Mesp1^BAC-mCherry
Gene Symbol and Name	Tg(RP24-353L5-mCherry)5Mik, transgene insertion 5, Michael I Kotlikoff
Gene Synonym(s)
Promoter	Anpep, alanyl (membrane) aminopeptidase, mouse, laboratory
Promoter	Ap3s2, adaptor-related protein complex 3, sigma 2 subunit, mouse, laboratory
Promoter	Mesp1, mesoderm posterior 1, mouse, laboratory
Promoter	Mesp2, mesoderm posterior 2, mouse, laboratory
Expressed Gene	Anpep, alanyl (membrane) aminopeptidase, mouse, laboratory
Expressed Gene	Ap3s2, adaptor-related protein complex 3, sigma 2 subunit, mouse, laboratory
Expressed Gene	Mesp1, mesoderm posterior 1, mouse, laboratory
Expressed Gene	Mesp2, mesoderm posterior 2, mouse, laboratory
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	mCherry is expressed in mesodermal cell lineages during early gastrulation.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The ~165 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP24-353L5 contains the entire mesoderm posterior 1 locus (Mesp1) as well as ~23 kbp centromeric flanking sequences (including a portion of the Wdr93 locus) and ~131 kbp distal flanking sequences (including the complete Mesp2, Anpep and Ap3s2 loci). Using homologous recombination/BAC recombineering, a construct containing the mCherry sequence, an SV40 polyadenylation signal, an SV40-puro-pA cassette and flanking vector sequences was all inserted into the ATG start site of the BAC Mesp1 gene (replacing the initiation codon of Mesp1 in exon 1). No other loci on the BAC were altered. mCherry expression is observed in mesodermal cell lineages during early gastrulation, including cardiac mesoderm and is consistent with endogenous Mesp1.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cardiovascular Research
- Heart Abnormalities
- Other
Cell Biology Research
- Channel and Transporter Defects
  - calcium
Developmental Biology Research
- Mesodermal Defects
  - Myogenesis Defects
Neurobiology Research
- Channel and Transporter Defects
  - calcium
- Optogenetic and Chemogenetic tools
  - Calcium sensor expressing strains
Research Tools
- Cardiovascular Research
- Cell Biology Research
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: endothelial cells

References

Additional - Tg(RP24-353L5-mCherry)5Mik related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :. MGI: J:101977

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: CHROMus line Mesp1BAC-mCherry

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Tg(RP24-353L5-mCherry)5Mik

Allele Symbol: Tg(RP24-353L5-mCherry)5Mik

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Tg(RP24-353L5-mCherry)5Mik related

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: CHROMus line Mesp1^BAC-mCherry