Etv5fl/fl floxed mutant mice may be useful for studying Etv5 transcriptional control during cell growth and differentiation, and stem cell self-renewal.
Xin Sun, University of California, San Diego
These Etv5fl/fl floxed mutant mice possess loxP sites flanking exons 10-11 of the ets variant gene 5 (Etv5) gene. Etv5 is a transcription factor required for cell growth and differentiation found in the brain, ovaries, kidneys, skeletal muscle, lung, and Sertoli cells in the testis. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 10-11 deleted in the cre-expressing tissues.
For example, when bred to mice deficient of Etv4 and subsequently to mice expressing Cre Recombinase in mesoderm-derived cells (See Stock No. 025520), anterior limb bud of the resulting offspring show ectopic sonic hedgehog expression and mice exhibit a preaxial polydactyly skeletal phenotype.
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette followed by a loxP site upstream of exon 10, and a second loxP site downstream of exon 11 of the ets variant gene 5 (Etv5) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Tg(ACTFLPe)9205Dym transgenic mice to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, and the resulting mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.1, Xin Sun|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Etv5, ets variant 5|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||An frt flanked neo cassette with a 3' loxP site was inserted upstream of exon 10 and an additional loxP site was inserted downstream of exon 11. Germ line, flp mediated recombination removed the neo cassette leaving exons 10 and 11 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Etv5fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #025534 in your Materials and Methods section.