129S.Cg-Crebbp^tm1Jvd/J

Stock number: 025532
Research areas: Research Tools

Description

Exon 9 of the mouse Crebbp gene is flanked by loxP sites in this conditional mutant strain that is useful in studies of tumor suppression and transcriptional regulation. Cre-mediated excision of the floxed region results in a knockout allele.

Detailed description

Crebbp (CREB binding protein, also known as CBP) is a protein acetyltransferase that is required for normal development and acts as a tumor suppressor. It interacts with a significant percentage of mammalian transcriptional regulatory proteins and mutations in the gene have been found in hematopoietic and epithelial tumors.

Exon 9 of the mouse Crebbp gene is flanked by loxP sites in this conditional mutant strain. Homozygous floxed mice are viable, fertile and have a normal lifespan. Cre-mediated excision of the floxed region results in a knockout allele, as confirmed by immunohistochemistry.

Development

A loxP site was introduced to intron 8 and a loxP-neomycin-HSV tk-loxP cassette was placed in intron 9 via homologous recombination in an unspecified embryonic stem (ES) cell line. Transient expression of cre recombinase in the ES cells excised the neomycin-HSV-tk cassette, leaving exon 9 flanked by loxP sites. Resultant chimeric mice were crossed with C57BL/6J animals, then to 129P2/Ola for 2 generations. Fertility/productivity issues precipitated 17 generations of backcrossing to 129S2/SvPasCrl by the donating laboratory.

Allele

Symbol: Crebbp^tm1Jvd
Name: targeted mutation 1, Jan M A van Deursen
MGI accession ID: MGI:3618462
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: CBP^flox
Marker symbol: Crebbp
Marker name: CREB binding protein
Marker synonyms: CBP; KAT3A; MKHK1; RSTS; RSTS1; RTS
Chromosome: 16
Strain of origin: Not Specified
Molecular note: A loxP recombination site was inserted in intron 8 and a floxed Neo-HSV tk selection cassette was inserted in intron 9. The selection cassette was then removed from correctly targeted ES cells by by transient expression of cre recombinase.