Exon 9 of the mouse Crebbp gene is flanked by loxP sites in this conditional mutant strain that is useful in studies of tumor suppression and transcriptional regulation. Cre-mediated excision of the floxed region results in a knockout allele.
Paul K. Brindle, St. Jude Children's Research Hospital
Crebbp (CREB binding protein, also known as CBP) is a protein acetyltransferase that is required for normal development and acts as a tumor suppressor. It interacts with a significant percentage of mammalian transcriptional regulatory proteins and mutations in the gene have been found in hematopoietic and epithelial tumors.
Exon 9 of the mouse Crebbp gene is flanked by loxP sites in this conditional mutant strain. Homozygous floxed mice are viable, fertile and have a normal lifespan. Cre-mediated excision of the floxed region results in a knockout allele, as confirmed by immunohistochemistry.
A loxP site was introduced to intron 8 and a loxP-neomycin-HSV tk-loxP cassette was placed in intron 9 via homologous recombination in an unspecified embryonic stem (ES) cell line. Transient expression of cre recombinase in the ES cells excised the neomycin-HSV-tk cassette, leaving exon 9 flanked by loxP sites. Resultant chimeric mice were crossed with C57BL/6J animals, then to 129P2/Ola for 2 generations. Fertility/productivity issues precipitated 17 generations of backcrossing to 129S2/SvPasCrl by the donating laboratory.
|Allele Name||targeted mutation 1, Jan M A van Deursen|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Crebbp, CREB binding protein|
|Strain of Origin||Not Specified|
|Molecular Note||A loxP recombination site was inserted in intron 8 and a floxed Neo-HSV tk selection cassette was inserted in intron 9. The selection cassette was then removed from correctly targeted ES cells by by transient expression of cre recombinase.|
Homozygous and heterozygous floxed mice are viable and fertile.
When using the 129S.Cg-Crebbptm1Jvd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025532 in your Materials and Methods section.