These CX3CL1cherry mice express mCherry in epithelial cell layers and some neurons, making these mice useful for studying leukocyte migration and trafficking, as well as integrin-independent adhesion and chemoattractive activity of monocytes.
Steffen Jung, Weizmann Institute of Science
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for the BAC CX3CL1cherry transgene are viable and fertile. The BAC has been modified to replace exon 1 of Cx3cl1 (chemokine (C-X3-C motif) ligand 1) with an mCherry fluorescent protein. CX3CL1 is the ligand for the chemokine receptor, CX3CR1. mCherry fluorescence is observed in mature neurons in the hippocampus, striatum, and cortical layer II and in epithelial cell layers. Specifically, expression is seen in the lung in alveolar and bronchial epithelial cells, including Clara cells. In the kidney, mCherry is also seen in tubular epithelial cells and glomeruli in the kidney, and in goblet cells and cells along the proximal and distal gastrointestinal tract.
When CX3CL1cherry mice are bred to B6.129P-Cx3cr1tm1Litt/J mice (Stock No. 005582), which express GFP in monocytes, dendritic cells, NK cells, and brain microglia, double mutant mice are useful when studying the interface of CX3CL1 and CX3CR1 in tissues and live animals.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24–147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to replace exon 1 of Cx3cl1 with an mCherry fluorescent protein. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. This BAC was microinjected into fertilized CB6F1 oocytes. Mice from founder line 1 were maintained by breeding transgenic mice with C57BL/6JOlaHsd inbred mice for at least 12 generations. The donating investigator reports that these mice were also bred to B6.129P-Cx3cr1tm1Litt/J mice (Stock No. 005582). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation, and the Cx3cr1tm1Litt allele was bred out of the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | mCherry fluorescence is observed in mature neurons in the hippocampus, striatum, and cortical layer II; alveolar and bronchial epithelial cells of the lung; tubular epithelial cells and glomeruli in the kidney; and in goblet cells and cells along the proximal and distal gastrointestinal tract. |
Allele Name | transgene insertion 1, Steffen Jung |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | CX3CL1cherry |
Gene Symbol and Name | Tg(Cx3cl1/mCherry)1Jung, transgene insertion 1, Steffen Jung |
Gene Synonym(s) | |
Promoter | Cx3cl1, chemokine (C-X3-C motif) ligand 1, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | mCherry fluorescence is observed in mature neurons in the hippocampus, striatum, and cortical layer II; alveolar and bronchial epithelial cells of the lung; tubular epithelial cells and glomeruli in the kidney; and in goblet cells and cells along the proximal and distal gastrointestinal tract. |
Strain of Origin | (BALB/c x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC(RP24-147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to replace exon 1 of Cx3cl1 with an mCherry fluorescent protein. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. |
When maintaining a live colony hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J (Stock No. 000664). The donating investigator has not attempted to make this strain homozygous.
When using the CX3CL1cherry mouse strain in a publication, please cite the originating article(s) and include JAX stock #025525 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Cx3cl1/mCherry)1Jung |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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