B6.Cg-Tg(Cx3cl1/mCherry)1Jung/J

Stock number: 025525
Product name: CX3CL1^cherry
Research areas: Cancer Research, Neurobiology Research, Research Tools

Description

These CX3CL1^cherry mice express mCherry in epithelial cell layers and some neurons, making these mice useful for studying leukocyte migration and trafficking, as well as integrin-independent adhesion and chemoattractive activity of monocytes.

Detailed description

Mice hemizygous for the BAC CX3CL1^cherry transgene are viable and fertile. The BAC has been modified to replace exon 1 of Cx3cl1 (chemokine (C-X3-C motif) ligand 1) with an mCherry fluorescent protein. CX3CL1 is the ligand for the chemokine receptor, CX3CR1. mCherry fluorescence is observed in mature neurons in the hippocampus, striatum, and cortical layer II and in epithelial cell layers. Specifically, expression is seen in the lung in alveolar and bronchial epithelial cells, including Clara cells. In the kidney, mCherry is also seen in tubular epithelial cells and glomeruli in the kidney, and in goblet cells and cells along the proximal and distal gastrointestinal tract.

When CX3CL1^cherry mice are bred to B6.129P-Cx3cr1^tm1Litt/J mice (Stock No. 005582), which express GFP in monocytes, dendritic cells, NK cells, and brain microglia, double mutant mice are useful when studying the interface of CX3CL1 and CX3CR1 in tissues and live animals.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (#RP24–147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to replace exon 1 of Cx3cl1 with an mCherry fluorescent protein. CCL22 and CCL17 sequences were removed from the BAC by restriction digest. This BAC was microinjected into fertilized CB6F1 oocytes. Mice from founder line 1 were maintained by breeding transgenic mice with C57BL/6JOlaHsd inbred mice for at least 12 generations. The donating investigator reports that these mice were also bred to B6.129P-Cx3cr1^tm1Litt/J mice (Stock No. 005582). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation, and the Cx3cr1^tm1Litt allele was bred out of the colony.

Allele

Symbol: Tg(Cx3cl1/mCherry)1Jung
Name: transgene insertion 1, Steffen Jung
MGI accession ID: MGI:5568579
Generation method: Transgenic
Attributes: Reporter
Synonyms: CX3CL1^cherry
Marker symbol: Tg(Cx3cl1/mCherry)1Jung
Marker name: transgene insertion 1, Steffen Jung
Chromosome: UN
Strain of origin: (BALB/c x C57BL/6)F1
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: mCherry fluorescence is observed in mature neurons in the hippocampus, striatum, and cortical layer II; alveolar and bronchial epithelial cells of the lung; tubular epithelial cells and glomeruli in the kidney; and in goblet cells and cells along the proximal and distal gastrointestinal tract.
Molecular note: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 120 kb BAC (RP24-147I16) containing the entire mouse Cx3cl1 (chemokine (C-X3-C motif) ligand 1) gene. This BAC was modified to replace exon 1 of Cx3cl1 with an mCherry fluorescent protein. CCL22 and CCL17 sequences were removed from the BAC by restriction digest.