Overview

Cx3cr1-Cre mice express Cre recombinase under the direction of the Cx3cr1 promoter in the mononuclear phagocyte system making them useful for fate-mapping studies of the monocyte and macrophage compartment, as well as microglia. These mice do not express endogenous Cx3cr1.

Donating Investigator

Steffen Jung, Weizmann Institute of Science

Genetic overview

Genetic Background	Generation
	N9+pN2F14 (2020-01-20 00:00:00)

Cx3cr1^{tm1.1(cre)Jung}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Cx3cr1	chemokine (C-X3-C motif) receptor 1

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

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Details

Detailed Description

The Cx3cr1-Cre knock-in/knock-out allele has expression of Cre recombinase directed by Cx3cr1 (chemokine (C-X3-C motif) receptor 1) promoter regions. These mice do not express endogenous Cx3cr1. CX3CR1 is a chemokine receptor expressed in the mononuclear phagocyte system, as well as microglia. Homozygous mice are viable and fertile. When Cx3cr1-Cre are bred with mice containing loxP-flanked sequence, cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring.

For example, when bred to B6.129X1-Gt(ROSA)26Sor^tm1(EYFP)Cos/J mice (Stock No. 006148), cre-mediated recombination results in yellow fluorescent protein expression in peritoneal, splenic and lung macrophages, and microglia and Kupffer cells. Some expression is also seen in circulating T and B lymphocytes, granulocytes and natural killer (NK) cells.

Depending on which floxed allele these mice are crossed to, significant rearrangement may occur in neurons as well as immune cells in the myeloid cell lineage. As adult neurons do not express CX3CR1, this is likely due to transient window of CX3CR1 promoter activity during neuronal development. For studies directed at understanding microglia function, use the conditional TAM-dependent CX3CR1-CreER mice (B6.129P2(C)-Cx3cr1^{tm2.1(cre/ERT2)Jung}/J; Stock No. 020940), in which no neuronal rearrangements are detected.

Development

Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC containing the entire mouse Cx3cr1 (chemokine (C-X3-C motif) receptor 1) gene. This BAC was modified to replace the coding exon of Cx3cr1 with a Cre recombinase gene and a loxP-flanked neomycin resistance cassette. This BAC was electroporated into C57BL/6N-derived embryonic stem (ES) cells. Chimeras were made by ES cell aggregation with eight-cell stage embryos and chimeric mice were bred with Tg(Pgk1-cre)1Lni mice to delete the neo cassette. Resulting offspring were bred to remove the cre-expressing transgene, and progeny were bred with C57BL/6JOlaHsd inbred mice for at least 9 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in the mononuclear phagocyte system.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Yona S; Kim KW; Wolf Y; Mildner A; Varol D; Breker M; Strauss-Ayali D; Viukov S; Guilliams M; Misharin A; Hume DA; Perlman H; Malissen B; Zelzer E; Jung S. 2013. Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. Immunity 38(1):79-91PubMed: 23273845 MGI: J:193038

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Genetics

Cx3cr1^{tm1.1(cre)Jung}

Allele Symbol: Cx3cr1^{tm1.1(cre)Jung}

Allele Name	targeted mutation 1.1, Steffen Jung
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cx3cr1^{tm1.1(cre)Jung}; targeted mutation 1.1, Steffen Jung
Gene Symbol and Name	Cx3cr1, chemokine (C-X3-C motif) receptor 1
Gene Synonym(s)	CMKDR1; GPR13; GPRV28; V28; CMKBRL1; CCRL1; Rbs11
Promoter	Cx3cr1, chemokine (C-X3-C motif) receptor 1, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in the mononuclear phagocyte system.
Strain of Origin	C57BL/6N
Chromosome	9
Molecular Note	A cre recombinase cassette with a floxed neo was introduced into a BAC by RedE/T recombineering, replacing the coding exon, prior to homologous recombination to target the gene in ES cells. Cre-mediated recombination removed the selection cassette.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Intracellular Signaling Molecules
- Inflammation
- Growth Factors/Receptors/Cytokines
Research Tools
- Cre-lox System
  - Cre Recombinase Expression

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Cx3cr1^{tm1.1(cre)Jung}/Cx3cr1⁺
B6.Cg-Cx3cr1

mammalian phenotype

no phenotypic analysis

References

Yona S; Kim KW; Wolf Y; Mildner A; Varol D; Breker M; Strauss-Ayali D; Viukov S; Guilliams M; Misharin A; Hume DA; Perlman H; Malissen B; Zelzer E; Jung S. 2013. Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. Immunity 38(1):79-91PubMed: 23273845 MGI: J:193038

Additional References

Rahmani W; Liu Y; Rosin NL; Kline A; Raharjo E; Yoon J; Stratton JA; Sinha S; Biernaskie J. 2018. Macrophages Promote Wound-Induced Hair Follicle Regeneration in a CX3CR1- and TGF-beta1-Dependent Manner. J Invest Dermatol 138(10):2111-2122PubMed: 29705291 MGI: J:266395
Gentek R; Ghigo C; Hoeffel G; Jorquera A; Msallam R; Wienert S; Klauschen F; Ginhoux F; Bajenoff M. 2018. Epidermal gammadelta T cells originate from yolk sac hematopoiesis and clonally self-renew in the adult. J Exp Med 215(12):2994-3005PubMed: 30409784 MGI: J:269027
Chen L; Strohmeier V; He Z; Deshpande M; Catalan-Dibene J; Durum SK; Moran TM; Kraus T; Xiong H; Faith JJ; Sodhi CP; Hackam DJ; Lira SA; Furtado GC. 2019. Interleukin 22 disrupts pancreatic function in newborn mice expressing IL-23. Nat Commun 10(1):4517PubMed: 31586069 MGI: J:281447

Additional - Cx3cr1^{tm1.1(cre)Jung} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Cx3cr1^{tm1.1(cre)Jung}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the B6J.B6N(Cg)-Cx3cr1^{tm1.1(cre)Jung}/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025524 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Cx3cr1<tm1.1(cre)Jung>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Cx3cr1tm1.1(cre)Jung

Allele Symbol: Cx3cr1tm1.1(cre)Jung

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cx3cr1tm1.1(cre)Jung/Cx3cr1+B6.Cg-Cx3cr1

mammalian phenotype

References

Additional References

Additional - Cx3cr1tm1.1(cre)Jung related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cx3cr1^{tm1.1(cre)Jung}

Allele Symbol: Cx3cr1^{tm1.1(cre)Jung}

Genotype: Cx3cr1^{tm1.1(cre)Jung}/Cx3cr1⁺
B6.Cg-Cx3cr1

Additional - Cx3cr1^{tm1.1(cre)Jung} related